慢性吗啡处理对大鼠交感神经节cAMP、cGMP水平的影响  

Effects of chronic morphine treatment on contents of cAMP and cGMP in sympathetic ganglia of rats

作　　者：何萍[1] 莫宁[1] 于洪波[1] 

机构地区：[1]广西医科大学药学院药理学教研室,广西南宁530021

出　　处：《中国药理学通报》2009年第1期109-112,共4页Chinese Pharmacological Bulletin

基　　金：广西自然科学基金青年基金资助项目(No桂科青0542045);广西医科大学博士启动基金资助项目(No308054)

摘　　要：目的观察慢性吗啡(Mor)处理对大鼠交感神经节——颈上神经节(SCG)环腺苷酸(cAMP)和环鸟苷酸(cGMP)含量的影响。方法剂量递增法连续皮下注射(sc)吗啡5 d建立慢性吗啡依赖大鼠模型,纳洛酮(Nal)催促戒断法和55℃热水甩尾试验判断模型依赖性和耐受性,125I放射性免疫测定法测定SCG中cAMP和cGMP的含量。结果①吗啡急性给药组大鼠SCG中cAMP含量较正常对照组降低(P<0.05);②吗啡依赖组大鼠SCG中cAMP含量回到正常对照组水平,并有增高趋势(与正常对照组比较,P>0.05;与吗啡急性给药组比较,P<0.05);③吗啡戒断组大鼠SCG中cAMP含量较正常对照明显增高(P<0.05);④各组大鼠SCG的cGMP含量差异无显著性(P>0.05)。结论慢性吗啡处理大鼠交感神经节cAMP存在适应性上调现象。Aim To study the effects of chronic mor- phine treatment on the contents of cAMP and cGMP in sympathetic ganglia, superior cervical ganglia （SCG） of rats. Methods The chronic morphine dependent model of rats was established by subcutaneous injection of morphine in gradually increasing doses for 5 days, the dependence and the tolerance of the model was estimated by naloxone precipitation test and 55℃ tail- flick trail test respectively. The contents of cAMP and cGMP in SCG were detected by means of 125I radioim- munoassay. Results （1） Compared with control group, the content of cAMP in SCG of morphine-acute group rats was descended （ P 〈 0. 05 ） ; （2） The content of cAMP in SCG of morphine dependent group rats was returned to the level of control group with an increased tendency （ compared with control, P 〉 0.05 ; compared with morphine-acute, P 〈 0.05 ）. （3） Compared with control group, the content of cAMP in SCG of morphine withdrawal group rats was increased （P 〈 0.05 ） ; （4） The content of cGMP in SCG was not significantly changed in every group （P 〉 0.05 ）. Conclusion There was an up-regulation of cAMP in sympa- thetic ganglia of chronic morphine treated rats.

关 键 词：吗啡 依赖 耐受 戒断 交感神经节 环腺苷酸 环鸟苷酸 

分 类 号：R-332[医药卫生] R322.85