DNA芯片技术在诊断恶性黑素瘤中的应用  

Applications of DNA chip technology in the diagnosis of melanoma

作　　者：辛崇美[1] 徐秀莲[1] 孙建方[1] 

机构地区：[1]中国医学科学院皮肤病研究所,南京210042

出　　处：《国际皮肤性病学杂志》2009年第1期13-15,共3页International Journal of Dermatology and Venereology

基　　金：基金项目：国家自然科学基金（30700727）;国家教委高校博士点基金（20070023113）

摘　　要：人类基因组序列的研究成功，为基因组功能分析奠定基础，而DNA芯片技术允许同时检测上千个基因，成为基因组功能分析的方法之一。从正常的黑素细胞、非典型痣、原位黑素瘤到侵袭性黑素瘤的发展过程，使恶性黑素瘤成为使用DNA芯片技术研究肿瘤发病机制的模型。使用DNA芯片技术根据恶性黑素瘤基因表达谱的不同，对恶性黑素瘤进行新的分型，发现恶性黑素瘤形成过程中的基因表达异常、染色体变异及与恶性黑素瘤转移相关的基因等。The successful sequencing of whole human genome provides a substantial basis for the research of gene function. DNA chip technology allows the simultaneous detection of thousands of genes, and has developed into a key method for functional genomics. The characteristic stepwise progression from normal pigment cells to atypical nevi, then to invasive primary melanoma and finally to cells with aggressive metastatic potential makes human melanoma an ideal tumor model for the study of human tumorigenesis. Based on the difference in gene expression profile in malignant melanoma, DNA chip technology could be applied to classify malignant melanoma, observe the abnormality in gene expression and chromosome in the development of malignant melanoma, and to detect malignant melanoma-related genes.

关 键 词：黑色素瘤 诊断 寡核苷酸序列分析 

分 类 号：R739.5[医药卫生—肿瘤]