蛇毒神经生长因子对高眼压下兔视网膜髓鞘碱性蛋白的影响  

The effects of venom nerve growth factor on myelin basic protein of retina in rabbits with elevated intraocular pressure

作　　者：钱道卫[1,2] 刘金华[1] 杜秀娟[1] 乔峰[1] 廖伟[1] 

机构地区：[1]广西医科大学第一附属医院眼科,南宁530021 [2]深圳恒生医院眼科,518102

出　　处：《眼科研究》2009年第1期10-13,共4页Chinese Ophthalmic Research

基　　金：国家自然科学基金项目资助(30460138)

摘　　要：目的通过对兔视网膜损伤后髓鞘碱性蛋白(MBP)的研究,观察蛇毒神经生长因子(vNGF)对慢性高眼压下兔视网膜神经节细胞(RGCs)的保护作用。方法24只大白兔右眼前房注入0.3%复方卡波姆溶液0.1mL,制成兔慢性高眼压模型后,随机分为vNGF治疗组和平衡盐液对照组,分别于眼压缓慢升高2周后右眼玻璃体腔内注入vNGF和平衡盐液;3d后重复注药1次。分别于最后1次给药后第5d、10d取材;采用病理图像分析仪计数RGCs数目;进行MBP免疫组织化学染色分析。结果前房注入复方卡波姆溶液后,眼压在1周内缓慢升高并维持到实验结束,平均眼压(32.93±6.33)mmHg。5d时实验组和对照组的RGCs分别为19.25±2.60和12.88±1.46;10d时实验组和对照组的RGCs分别为16.84±1.52和7.63±1.72,差异均有统计学意义(P<0.05)。对照组MBP表达高于实验组。结论vNGF能降低高眼压后视网膜MBP的含量,提高RGCs的存活数量,对RGCs损伤后的修复有明显的促进作用。Objective Myelin basic protein （MBP） is key protein in sustaining structure and function stabilization of neuron and nerve fiber myelin sheath. Our previous study demonstrated that venom nerve growth factor（vNGF） has a protection on acute optical nerve damage of rat. Present study was to observe the protective effect of vNGF on retinal ganglion ceils（RGCs） in rabbits with elevated intraoeular pressure（ IOP）. Methods Chronic ocular hypertention was induced by injection of 0.3% carbomer into anterior chamber of the right eyes in 24 rabbits. The rabbits were divided into two groups randomly. After elevation of IOP continuously for 2 weeks,vNGF was injected into the vitreous of the right eyes of rabbits in the experimental group, and balance salt solution was injected in the control group with matched-IOP for 2 times at a 3-day interval. The eyeballs of rabbits were enucleated and the section samples were prepared at 5 days and 10 days after the last injection. The numbers of the RGCs were counted by using image analysis system. The expression of MBP in rabbit retina was detected by regular HE stain and immunohistochemistry under the light microscope. Results Chronic ocular bypertention models were successfully established with the mean IOP （32.93 ±6.33） mmHg through the experimental period. The numbers of RGCs were 19.25±2.60 and 16.84±1.52 in the experimental group and 12.88±1.46 and 7.63 ±1.72 in control group at the 5th days and 10th day after injection of 0. 3% carbomer, showing statistically significant differences between them （P 〈 0.05 ）. The expression of MBP in rabbit retina was more prominent in control group than in experimental group. Conclusion vNGF can reduce the expression of MBP in retina and therefore increase the survival rate of RGCs. vNGF may have a protective effect on the prevention of RGCs from degeneration.

关 键 词：蛇毒神经因子 视网膜神经节细胞 眼压 髓鞘碱性蛋白 

分 类 号：R774[医药卫生—眼科]