应用抑制性消减杂交筛选卵巢癌紫杉醇耐药差异表达基因  

Screening differentially expressed genes of Taxol-resistant in ovarian cancer by suppression subtractive hybridization.

作　　者：赵晋[1] 李红霞[1] 丁翔[2] 

机构地区：[1]北京世纪坛医院妇产科,北京100038 [2]北京市海淀区妇幼保健院

出　　处：《现代妇产科进展》2008年第11期801-804,共4页Progress in Obstetrics and Gynecology

基　　金：首都医学发展科研基金(重点学科)资助项目(No:ZD199915)

摘　　要：目的:应用抑制性消减杂交技术研究人卵巢癌紫杉醇耐药细胞株OC3/Tax300与其亲本细胞OC3(敏感株)之间基因表达的差异,筛选耐药相关基因,探讨基因表达差异与卵巢癌耐药的相关性。方法:以卵巢癌紫杉醇耐药细胞株OC3/Tax300 mRNA为检测子(tester),以其亲本细胞OC3(敏感株)mRNA为驱赶子(driver),构建cDNA消减文库。随机挑取文库克隆测序,所得结果在GenBank中做同源性比较分析。结果:成功构建了卵巢癌紫杉醇耐药细胞株的特异性cDNA消减文库。从文库中选取阳性克隆,其中76个测序成功,再随机选取36个序列与GenBank数据库进行初步比对,8个可能为新基因,1个为蛋白序列,另27个来源于16个已知基因,这些差异表达基因主要涉及细胞代谢、信号转导、细胞骨架、凋亡、蛋白翻译合成、发育等相关基因。结论:部分基因表达差异与卵巢癌紫杉醇耐药有关。Objective ：To investigate the differential gene expression of taxol-resistant in ovarian cancer cell line OC3/Tax300 compared with its parental cell OC3 （sensitive lines） using suppression subtractive hybridization;to study its relationship with Drug-resistance and its mecha- nism. Method：To construct subtractive eDNA library of taxol-resistant ovarian cancer cell line from the mRNA of OC3/Tax300 （tester） and OC3 （driver）,partial positive clones in the library were randomly selected,sequenced and then analyzed with the BLAST software in GenBank. Resnit：Subtractive eDNA library of taxol-resistant ovarian cancer cell line was constructed with 76 clones successfully sequenced, among which 36 clones were selected randomly and compared with the GenBank Database. 27/36 sequence had a high similarity to 16 known genes;i/36 was pro- tein sequence,while 8/36 failed to match with the homologous genes in Genbank. These subtrac- tive genes were mainly related to cellular energy process, signaling pathway, cytoskeleton, apopto- sis,protein synthesis, growth and development, etc. Conclusion： Part of these differentially ex- pressed genes is related to the mechanisms of taxol-resistance of ovarian cancer.

关 键 词：卵巢肿瘤 紫杉醇耐药 基因差异表达 抑制性消减杂交 

分 类 号：R737.33[医药卫生—肿瘤]