乳腺癌组织Ezrin表达及其与临床病理特征关系的研究  

Expression of Ezrin and its relationship with clinicopathological characteristics in breast cancer

作　　者：余慧萍[1] 樊祥山[2] 张东生[1] 孟凡青[2] 周强[2] 吴鸿雁[2] 

机构地区：[1]东南大学基础医学院病理与病理生理学系,江苏南京210009 [2]南京大学医学院附属鼓楼医院病理科,江苏南京210008

出　　处：《中华肿瘤防治杂志》2008年第19期1441-1444,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30371830);南京市科技发展计划项目(200601050);南京市医学科技发展课题(YKK05094)

摘　　要：目的:研究Ezrin表达与乳腺癌临床病理特征的关系。方法:应用组织芯片技术和免疫组织化学方法检测117例浸润性乳腺癌和41例乳腺良性增生中,Ezrin、ER、PR、c-erbB-2、MMP-2及MMP-9的表达。结果:Ezrin在91.89%(34/37)的乳腺良性增生中表达于导管腔缘上皮顶膜,在细胞质内无表达;在76.99%(87/113)的乳腺癌组织中,Ezrin表现为细胞质中弥散表达,未见顶膜表达,两者差异有统计学意义,P=0.000。在乳腺癌组织中,Ezrin表达与肿瘤最大直径、TNM分期、淋巴结转移以及基质细胞中MMP-2和MMP-9的表达呈正相关,P值分别为0.016、0.002、0.036、0.007和0.002;而与年龄、组织学分级、ER、PR、c-erbB-2、肿瘤细胞中MMP-2及MMP-9表达无明显相关性。结论:Ezrin在乳腺良恶性病变中有明显不同的亚细胞定位,其表达情况对乳腺病变性质的判断具有重要价值。高表达Ezrin的乳腺癌具有更高的侵袭性和淋巴结转移能力,在乳腺癌组织中检测Ezrin的表达能为患者的预后和淋巴结转移风险评估提供重要参考。OBJECTIVE： To investigate the expression of Ezrin and its relationship with clinieopathological characteristics in breast cancer. METHODS： By the tissue microarray technique and immunohistochemical method,expression of Ezrin, ER, PR, c-erbB-2, MMP 2 and MMP-9 was detected in 117 cases of invasive breast carcinoma and 41 cases of benign breast hyperplasia. RESULTS： The subcellular distributions of Ezrin in benign and malignant mammary tissues were significantly different, P = 0. 000. Ezrin staining in most （91.89%, 34/37） benign breast epithelium localized at the apical, and no cytoplasmic staining was observed, but in 76.99% （87/113）of breast cancer cases, it localized in the cytoplasm and no apical staining was observed. In breast cancer cases, Ezrin expression in tumor cells was positively related with larger tumor size （P=0. 016）, higher TNM stage （P=0. 002）, lymph node metastases （P=0. 036）, stronger ex pressionof MMP-2（P=0. 007）, and MMP-9 （P=0.002） in stromal cells. There were no further associations between Ezrin expression and any other clinico-pathological features. CONCLUSIONS： Subcellular distribution of Ezrin change obviously in benign and malignant lesions of breast, which is of aid in estimating the characteristics of breast lesions. The higher expression level of Ezrin in breast carcinoma suggests stronger ability of invasion and lymph node metastasis.

关 键 词：乳腺肿瘤/病理学 EZRIN 淋巴结转移 组织芯片 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]