干扰素α对BEL-7402细胞生长与端粒酶活性影响的观察  

Anti-proliferation effect of interferon-α on hepatocellular carcinoma BEL-7402 cells and its influence on hTERT-mRNA expression and telomerase activity

作　　者：刘加军[1] 张俊峰[2] 林东军[1] 陆敏强[2] 蔡常洁[2] 

机构地区：[1]中山大学附属三院血液内科,广东广州510630 [2]中山大学附属三院肝移植中心,广东广州510630

出　　处：《中华肿瘤防治杂志》2008年第20期1532-1535,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30772782);教育部新世纪优秀人才支持计划资助项目(NCET-06-0721);广东省中医药局课题(2007103)

摘　　要：目的:探讨干扰素α(IFN-α)对肝癌BEL-7402细胞的生长抑制作用及其对肝癌细胞端粒酶hTERT-mRNA表达水平和端粒酶活性的影响。方法:以不同浓度的IFN-α作用于体外培养的肝癌BEL-7402细胞,MTT法检测细胞生长抑制率,应用流式细胞仪及ho-echst33258荧光染色法观察细胞凋亡;并对细胞凋亡前后的端粒酶hTERT-mRNA表达水平及端粒酶活性进行检测。结果:IFN-α可显著抑制肝癌BEL-7402细胞的生长及诱导细胞发生凋亡,呈现出明显的量-效与时-效关系。在细胞凋亡过程中端粒酶hTERT-mRNA的表达水平及端粒酶活性均显著下降。结论:IFN-α能抑制BEL-7402细胞的生长并诱导细胞发生凋亡,降低端粒酶hTERT-mRNA的表达水平及端粒酶活性可能是其重要作用机制。OBJECTIVE： To investigate the anti-proliferation effect of interferon α on hepatocellular carcinoma BEL-7402 cells and its influence on hTERT mRNA expression and telomerase activity. METHODS： BEL 7402 cells in culture medium in vitro were treated with different concentrations of interferon-α. The inhibitory rates of cells were measured by MTT assay, apoptosis was obsevered by flow cytometry（FCM） and hoeehst 33258 fluorescence staining, and bTERT-mRNA expression as well as the activity of telomerase were detected before and after apoptosis occurred. RESUL3S： Interferon α inhibited the growth of BEL-7402 cells and caused apoptosis remarkably, the suppression was both in a time and dose-dependent manner, and hTERT-mRNA expression as well as the activity of telomerase were decreased significantly. CONCLUSION： Interferon-α can inhibit the growth and induce apoptosis of BEL-7402 cells significantly, and decreasing hTERT-mRNA expression and telomerase activity of BEL-7402 cells may be its most important mechamisms.

关 键 词：肝肿瘤 干扰素 端粒酶 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤] R73-362[医药卫生—临床医学]