基因表达芯片分析筛选胃癌转移相关基因  

Pathway analysis of gene microarray selecting gastric cancer metastasis-related genes

作　　者：徐凌[1] 朱秀[1] 殷建华[2] 张敏峰[3] 赵晋丰[2] 曹广文[2] 邓松华[1] 

机构地区：[1]安徽医科大学病理生理学教研室,安徽合肥230032 [2]第二军医大学流行病学教研室,上海200433 [3]第二军医大学附属长海医院普通外科,上海200433

出　　处：《中华肿瘤防治杂志》2008年第20期1568-1571,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：安徽省自然科学基金(050430706)

摘　　要：目的:筛选高侵袭型胃癌转移相关基因,探讨其在胃癌发生发展中可能通路的分子水平机制。方法:分别以年龄相近、性别相同的3例肝转移胃癌患者标本的转移灶和原发灶为实验组和对照组做高通量cDNA表达谱芯片,初选3张芯片共同上、下调基因;利用pathway V1.0分析软件寻找初筛基因中存在多种可能影响胃癌发生发展及其转归的分子通路;通过RT-PCR测定数例转移胃癌病例原发和转移灶中相关基因的mRNA转录水平。结果:通路分析筛选出来的上调基因CLDN1和下调基因UGT1A10在6组病例中经RT-PCR定性检测全部与分析结果相符。结论:pathway在芯片初选相关基因的基础上发现与疾病相关的分子机制通路及相关基因,为研究胃癌转移基因提供依据和参考。OBJECTIVE： To select the metastasis-related genes in gastric cancer with high invasion risk and discuss the probable molecule mechanism of pathway in the process of gastric cancer. METHODS： Three paires of metastasized liver tissues and carcinoma in situ tissues of stomach （men in the same stage of age） were used as experimental group and control group respectively to establish high-flux cDNA expression spectrum chips. The up-regulating or down-regulating genes were chosen simultaneously in all the three chips. The software of Pathway V1.0 was applied in the investigation of multi-kinds of molecular pathways which might influence the genesis and progression of stomach carcinoma in the already selected genes. RT-PCR was employed to determine the transcription of mRNA to the related genes in several cases of metastasis stomach carcinoma in the primary and metastasis tissues. RESULTS： The up-regulating CLDN1 gene and down-regulating UGT1A10 gene selected by the pathway analysis in this study completely accorded with the qualitical detection of 6 cases by RT-PCR. CONCLUSIONS： The pathway analysis can find molecule pathways and related-genes on the basis of the primarily selected correlation genes in the chip, providing the evidences and references for the gene study on gastric cancer with metastasis.

关 键 词：胃肿瘤 肿瘤转移 寡核苷酸序列分析 

分 类 号：R735.2[医药卫生—肿瘤]