APE1在宫颈癌的表达及其与锎-252中子放疗预后的关系  

The Expression of APE1 and Its Correlation with Prognostic Significance after ^(252)Cf Radiotherapy in Cervical Cancer

作　　者：卿毅[1] 王东[1] 雷新[1] 向德兵[2] 李梦侠[1] 李增鹏[2] 单锦露[1] 

机构地区：[1]第三军医大学大坪医院肿瘤中心,重庆400042 [2]第三军医大学大坪医院病理科,重庆400042

出　　处：《四川大学学报（医学版）》2009年第1期125-128,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30472004);重庆市自然科学基金项目(批准号2004BB5246);第三军医大学科研创新基金(批准号2007XG36)资助

摘　　要：目的探讨脱嘌呤/脱嘧啶核酸内切酶(APE1)基因在宫颈癌中的表达情况及其与临床病理和锎-252中子放射治疗预后的关系。方法应用免疫组织化学法检测10例正常宫颈组织、15例子宫颈上皮内肿瘤(CIN)组织、89例宫颈癌组织(接受锎-252中子刀放疗)中APE1的表达,并分析其与宫颈癌临床病理及患者预后的关系。结果宫颈癌组织APE1表达水平明显高于正常宫颈组织和CIN病例(P<0.01)。APE1在正常宫颈组织和CIN病例均呈胞核表达,宫颈癌组织中APE1呈胞核表达(59例)、单纯胞浆表达(8例)或核浆共同表达(22例)。APE1表达强度与宫颈癌FIGO分期、病理分级和淋巴结转移情况有关(P<0.05),与年龄和病理分型无关。APE1亚细胞定位情况与FIGO分期、病理分级有关(P<0.01),与淋巴结转移情况无关。生存分析显示在APE1核表达组(中位生存时间70.9月)和APE1低表达组(中位生存时间75.8月)的生存时间明显长于APE1浆表达组(中位生存时间57.8月)和APE1高表达组(中位生存时间56.5月)(P=0.025,0.001)。结论APE1胞质异位表达可能与宫颈癌的发生、发展相关,APE1亚细胞定位和表达水平对锎-252中子放射治疗宫颈癌的疗效有提示作用。Objective To investigate the expression feature of the apurinic/apyrimidinic endonuclease （APE1） and its correlation with clinicopathology and prognostic significance after ^252 Cf radiotherapy in cervical cancer. Methods The expression of APE1 was detected by immunohistochemistry technique in 89 cases of cervical cancer （treated by ^252Cf）, 15 cases cervical intraepithelial neoplasia （CIN） and 10 cases of normal cervical tissue, and its association with clinicopathological data as well as prognosis were analyzed. Results The expression of APE1 in cervical cancer is higher significantly than that in normal cervical tissue and CIN （P〈0.01）. In normal cervical tissue and CIN, the APE1 express was located in the nucleus. In cervical cancer, the APE1 express was located in the nucleus （59）, cytoplasm （8） or nucleus and cytoplasm （22）, the location of APE1 was related with FIGO stage and pathological grade （P〈0.01）, and not related with lymph node metastasis. The level of APE1 express related with FIGO stage, pathological grade and lymph node metastasis （P〈0. 05）, and not related with age and pathological type. The Kaplan-Meier survival analysis demonstrated that the survival time of the group of APE1 nucleus expression （median survival time is 70. 9 months） and the group of APE1 low expression （median survival time is 75.8 months） is longer significantly than that of the group of APE1 cytoplasm expression （median survival time is 57.8 months） and the group of APE1 high expression （median survival time is 56.5 months） （P=0. 025, 0. 001）. Conclusion The dystopic express of APE1 might play a pivotal role in carcinogenesis and progression of cervical cancer, and the express of APE1 might estimate the prognosis after ^252Cf radiotherapy.

关 键 词：脱嘌呤/脱嘧啶核酸内切酶 锎-252中子 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]