人类胃癌细胞高侵袭亚系的筛选及其生物学特性研究  

Screening and characterization of gastric carcinoma subcell line with higher invasive potential

作　　者：周志姣[1,2] 朱建思[1] 欧翔[2] 周建国[1] 王成昆[3] 彭芳[1] 全细云[1] 周智秀 

机构地区：[1]南华大学肿瘤研究所,湖南衡阳421001 [2]韶关学院医学院基础医学部 [3]中南大学肿瘤研究所 [4]郴州市精神病医院

出　　处：《肿瘤研究与临床》2009年第1期7-10,13,共5页Cancer Research and Clinic

基　　金：广东省韶关学院医学院科研计划（E08010）;广东省韶关市医药卫生科研计划（Y09067）

摘　　要：目的采用Transwell小室筛选胃癌细胞高侵袭亚系并研究其生物学特性。方法利用Transwell小室筛选高侵袭力胃癌细胞亚系，通过HE染色、Western blotting、Transwell小室模型、免疫组织化学法及生长曲线研究亚系的生物学特性。结果利用Transwell小室反复筛选10次，从MKN-28细胞中筛选出高侵袭力胃癌细胞亚系MKN-28S10。母亚两系细胞形态上无明显差异，但亚系中E—cadherin和TIMP-1蛋白表达同母系相比显著降低（P〈0．05），显微镜下细胞计数示亚系迁移至膜背面的数量（100．25±0．50／视野）较母系（61．75±2．06／视野）明显增多（P〈0．05），细胞运动能力明显增强；免疫组织化学结果显示，亚系中NM23-H1的表达明显低于母系（P〈0．05）；亚系体外生长速度快于母系。结论MKN-28S10细胞较MKN-28细胞具有更高侵袭力和更强增生能力。Objective To study a gastric carcinoma subcell line with higher invasive potential and its biologic characteristics screened by a transwell chamber. Methods Transwell chamber was used for the selection of tumor subline. The biological characteristics of the cell lines were studied with optics microscope, Western blotting, Transwell, immunohistochemical staining and growth curve. Results A gastric carcinoma cell subeell line （named MKN-28S10） was established from its parent cell line MKN-28 with higher invasive potential. MKN-28S10 showed essentially the same morphous as MKN-28. The expression of E-cadherin and TIMP-1 decreased significantly in the screened subcell line（P 〈0.05）. The expression of NM23-H1 in MKN- 28S10 was significantly lower than that in MKN-28 （P 〈0.05）. Compared with MKN-28 （61.75±2.06 per vision）, the migrative ability of passing through the membrane Millipore（100.25±0.50 per vision） was obviously increased in MKN-28S10（P〈0.05）. Compared with MKN-28, the growth rate of MKN-28SI0 was increased obviously. Conclusion MKN-28S10 cell has stronger invasive ability and more powerful proliferation than that of its mother line MKN-28.

关 键 词：胃肿瘤 肿瘤浸润 TRANSWELL 筛选 

分 类 号：R735[医药卫生—肿瘤]