苏木精修饰瘤苗加入不同佐剂对HepA的抑瘤作用  

Immunological effect of tumor cell vaccines to hepatoma A （HepA） treated by different adjuvant of Haematoxylin

作　　者：杨芳[1] 张聪[2] 白喜花[3] 赵娜[3] 闫磊[3] 杨永明[3] 任连生[3] 

机构地区：[1]山西省肿瘤医院药剂科,太原030013 [2]山西省肿瘤医院预防保健科,太原030013 [3]山西省肿瘤研究所动物实验室

出　　处：《肿瘤研究与临床》2009年第1期11-13,共3页Cancer Research and Clinic

摘　　要：目的观察苏木精修饰的肿瘤细胞瘤苗对小鼠移植性肝癌HepA的抑瘤作用。方法小鼠HepA细胞经0．1％的苏木精处理后，制备成三种瘤苗：弗氏完全佐剂瘤苗、弗氏不完全佐剂瘤苗、不含佐剂的肿瘤细胞瘤苗。分别用三种瘤苗免疫小鼠5次后，以活的HepA细胞1×10^5／鼠腹腔接种攻击，观察各组动物的平均生存时间，比较三种瘤苗对荷瘤动物的免疫保护作用，以等量生理盐水代替瘤苗作对照组。结果对照组小鼠的平均生存时间为（23．30±1．24）d；弗氏完全佐剂组为（43．90±15．20）d（P〈0．02）；弗氏不完全佐剂组为（39．60±13．77）d（P〈0．05）；不含佐剂组为（38．40±12．54）d（P〈0．05）。与对照组比较，各组动物的生命延长率分别为88．41％、69．96％、64．81％。结论经苏木精修饰的小鼠肝癌瘤苗对荷瘤小鼠可产生显著的保护作用；弗氏佐剂对肿瘤细胞瘤苗免疫效果有增强的作用。Objective To observe the immunological effect of tumor cell vaccines to mouse hepatoma A（HepA） treated by Haematoxylin. Methods HepA cell was treated by 0.1% Haematoxylin and made into three vaccines： HepA vaccine with complete Freund＇ s adjuvant, HepA vaccine with incomplete Freund＇ s adjuvant; and HepA vaccine without any adjuvants. Five times of immunization were given the grouped mice with the above three vaccines, then active HepA cells （1 ×10^5 for each mouse） were inoculated by intraperitoneal injection to attack them; reckoning the mean survival time （MST） of the grouped mice, comparing the immunoprotective action of the three vaccines to the tumor-bearing mice. Those mice only receiving normal saline （equal volume to the vaccine） were as a control group. Results MST of control group was （23.30±1.24） day; MST of mice receiving H22 vaccine with complete adjuvant was （43.90 ± 15.20） day （P 〈0.02）; MST of mice receiving H22 vaccine with incomplete adjuvant was （39.60 ±13.77） day （P 〈0.05）; and MST of mice receiving HepA vaccine without any adjuvant was （38.40±12.54） day （P 〈0.05）; As compared with the control group, the three treated groups showed a life-lengthening rate （LLR） 88.41%, 69.96 % and 64.81% respectively. Conclusion The vaccines treated by Haematoxylin give a marked immunoprotective action to those tumor-bearing mice. The HepA vaccine＇ s immunological effect is increased by the Freund＇s adjuvant （complete or incomplete）.

关 键 词：苏木精 细胞系 肿瘤 肝肿瘤 实验性 免疫 

分 类 号：R73[医药卫生—肿瘤]