蛋白芯片技术对甲胎蛋白阴性肝癌诊断的意义  

Discovery of biomarkers from serum in hepatocellular carcinoma using protein chip technique

作　　者：叶韵斌[1] 陈慧菁[1] 周东[2] 郝明志[3] 刘枋[1] 余家密[1] 李星[1] 林海澜[3] 陈强[3] 

机构地区：[1]福建省肿瘤医院肿瘤免疫学研究室,福州350014 [2]福建省肿瘤医院外科,福州350014 [3]福建省肿瘤医院内科,福州350014

出　　处：《肿瘤研究与临床》2009年第1期17-19,共3页Cancer Research and Clinic

基　　金：福建省卫生教育联合攻关计划（WKJ2005-2-015）

摘　　要：目的探讨表面增强激光解吸离子化飞行时间质谱（简称SELDI）技术在甲胎蛋白（AFP）阴性肝癌患者中的诊断价值。方法应用美国Ciphergen公司SELDI仪和CM10芯片检测57例血清AFP阴性（〈20ug／L）肝癌患者和55例健康对照外周血清。采用Biomarker Wizard软件分析，筛选出特征性的蛋白峰，结合临床病理资料分析该蛋白峰的诊断价值。结果筛选出的特异蛋白峰质荷比为4．2×10^3,4．1×10^3、6．7×10^3．5．7×10^3、6．5×10^3．6．9×10^3,5．8×10^3，利用差异蛋白峰对17例AFP阴性的肝癌患者和13例健康对照进行盲筛，其灵敏度和特异度分别为88．23％和92.31％。筛选出的特异蛋白峰与患者年龄、性别、肿瘤大小以及是否合并肝硬化等临床资料无关。结论应用SELDI技术筛选肝癌患者血清特异性肿瘤标志物的方法快速、有效，对血清AFP阴性患者的正确诊断有较大的辅助作用。Objective To screen serum biomarkers in patients with hepatocellular carcinoma by using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry（SELDI-TOF-MS）technique and to evaluate its clinical implication in the patients whose alpha-fetoprotein were negative in the sera. Methods Proteomic spectra were generated by mass spectroscopy in 1 12 cases, including 57 cases AFP- negative hepatocellular carcinoma,and 55 cases of healthy control. The consequence was analyzed and the characteristic proteomic peaks was selected by using Biomarker Wizard. Results Seven low expressed potential biomarker were indentified with the mass-to-charge ratio of 4.2×10^3, 4.1×10^3, 6.7×10^3, 5.7×10^3, 6.5× 10^3, 6.9×10^3, 5.8×10^3. The sensitivity for diagnosing hepatic cancer were 88.23 % and specificity was 92.31%. These peaks were not correlated with age, sex, tumor mass size, pathology grading and cirrhosis in hepatocellular carcinoma. Conclusion SELDI-TOF-MS offers a unique platform for the proteomic detection of hepatocellular carcinoma.It also offers an auxiliary diagnosis method to the patients whose alpha-fetoprotein are negative in the serum.

关 键 词：肝细胞性肝癌 肿瘤标志 生物学 SELDI 甲胎蛋白类 

分 类 号：R735[医药卫生—肿瘤]