抗痨药物致大鼠肝脏损害的电镜研究  

ELECTRON MICROSCOPIC STUDIES ON HEPATIC LESION INDUCED BY ANTITUBERCULAR DRUGS

作　　者：李登瑞[1] 胡建军[1] 查月芳[1] 高官聚[1] 吴树才[1] 黄凌慧[2] 

机构地区：[1]河北省胸科医院内科,河北石家庄050041 [2]中国人民解放军总后勤部武汉干休所卫生所,湖北武汉430035

出　　处：《河北医科大学学报》2009年第1期16-18,I0001,I0002,共5页Journal of Hebei Medical University

摘　　要：目的以透射电镜观察不同抗痨药物对SD大鼠肝脏超微结构的影响。方法健康成年SD大鼠（清洁级）152只，体质量250-300g，雌雄各半，随机分为8组。其中7组为使用不同药物的观察组；1组为使用生理盐水的对照组。每天对各组大鼠进行一次灌胃，10d后处死大鼠，分离肝脏，戊二醛固定后做电镜观察。结果肝细胞部分线粒体溶解，粗面内质网脱颗粒，滑面内质网池扩张，细胞内脂肪颗粒增多，糖原颗粒显著增多；细胞核周水肿；肝细胞间胶原纤维增生。结论异烟肼、利福平、吡嗪酰胺均可引起大鼠肝脏的损害，多种药物联用比单药应用损害更明显；随着药物应用种类的增多，肝脏损害逐渐加重；吡嗪酰胺组比其他单用药组肝脏毒性更大。Objective To study the ultrastructure of hepatic lesion induced by antitubercular drugs. Methods One hundred and fifty two healthy adult rats,weighing about 250 -300 g,half male and half female,were randomly divided into 8 groups. Seven groups are given different drugs and the other one is given NS. Results Some mitochondrions in the liver cells dissolve, rough surfaced endoplasmic reticulum degranulation, smooth surfaced endoplasmic reticulum pool dilatation, the fat particles in the cell increase, hepatin particle apparently increase, dropsy appear around the karyons; collagenous fiber between the liver cells hyperplasia. Conclusion [isonicotinyl hydrazide, INH （H）], [rifampicin, RFP（R）] and [pyrazinamide, PZA （Z）] can lead to the damages of hepatic tissue, especially in the usage of multiple drugs; the degree of hepatic injury is increased with the numbers of drugs used; the toxicity of PZA on liver is greater than that of other antitubercular drugs.

关 键 词：抗结核药 肝细胞 大鼠 

分 类 号：R361.2[医药卫生—病理学]