Rapid Estimation of Enantioselectivity in Lipase-catalyzed Resolution of Glycidyl Butyrate Using pH Indicator  

作　　者：WANG Ping WANG Lei WANG Li-cheng LI Chun-yuan WANG Ren MIAO Qing-hua YANG Ming WANG Zhi 

机构地区：[1]Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education [2]College of Life Science, Jilin University, Changchun 130021, P. R. China [3]Department of Breast Surgery, The First Hospital of Jilin University, Changchun 130021, P. R. China

出　　处：《Chemical Research in Chinese Universities》2009年第1期72-75,共4页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos.20072011, 20272017);the Foundation of Research Program of Jilin University, China.

摘　　要：A simple method for rapid estimation of the enantioselectivity of lipase in resolution of chiral esters is described. The enantioselectivity of lipase can be estimated rapidly through comparing the difference of hydrolysis rates for the racemic ester and its slow reacting enantiomer under the same condition because the difference mainly depends on the enantioselective ratio（E values）. The higher the enantioselectivity of enzyme, the larger the difference of hydrolysis rate. The bromothymol blue（BTB） can be used as pH indicator for microplate reader to monitor the formation of acid in lipase-catalyzed hydrolysis of esters. This method has been successfully used to rapidly estimate the enantioselectivity of several lipases in the resolution of glycidyl butyrate.A simple method for rapid estimation of the enantioselectivity of lipase in resolution of chiral esters is described. The enantioselectivity of lipase can be estimated rapidly through comparing the difference of hydrolysis rates for the racemic ester and its slow reacting enantiomer under the same condition because the difference mainly depends on the enantioselective ratio（E values）. The higher the enantioselectivity of enzyme, the larger the difference of hydrolysis rate. The bromothymol blue（BTB） can be used as pH indicator for microplate reader to monitor the formation of acid in lipase-catalyzed hydrolysis of esters. This method has been successfully used to rapidly estimate the enantioselectivity of several lipases in the resolution of glycidyl butyrate.

关 键 词：pH indicator ENANTIOSELECTIVITY LIPASE Glycidyl butyrate 

分 类 号：O623.624[理学—有机化学]