微RNA18与B细胞易位基因2在肝癌细胞中差异表达相关性的研究  

The relationship between microRNA-18 and BTG2 in the carcinogenesis of hepatocellular carcinoma

作　　者：李琼[1] 王阁[1] 张志敏[1] 杨志祥[1] 单锦露[1] 陈川[1] 许文[1] 雒喜忠[1] 王东[1] 

机构地区：[1]第三军医大学野战外科研究所大坪医院肿瘤中心,重庆400042

出　　处：《中华肝脏病杂志》2009年第1期42-45,共4页Chinese Journal of Hepatology

基　　金：基金项目：国家自然科学基金（30570410、30370341）;全国优秀博士专项基金（200261）;院所135课题

摘　　要：目的研究微RNA18（miR18）对肝癌细胞HepG2基因表达谱的影响，预测其靶基因，初步探讨miR-18与抗增殖基因B细胞易位基因2（BTG2）两者在肝癌发生过程中差异表达的相关性。方法利用基因表达谱芯片技术筛选HepG2的微RNA差异表达谱，应用生物信息学方法预测表达明显上调的miR-18的靶基因，初步筛选出直接调控的靶基因为抗增殖基因BTG2；应用RT-PCR和Northernblot法分析BTG2正常与肝癌组织中的表达情况。结果生物信息学分析结果显示miR-18分子调控的下游靶基因有609个，涉及细胞增殖、分化和凋亡、转录调节等众多生理和病理过程；抗增殖基因BTG2在肝癌组织和细胞中呈明显低表达。结论miR-18在肝癌细胞中表达明显上调，并可能负性调控抗增殖基因BTG2在肝癌细胞中的表达，两者共同在肝癌细胞增殖方面发挥着重要作用。Objective To study the difference ofmicroRNA expression between HepG2 cells and L02 ceils, and to identify the target genes ofmicroRNA-18 （miR-18）. Methods The differentially expressed miRNAs between HepG2 cells and L02 cells were identified by miRNA chip. Target genes ofmiR-18 were predicted bioinformatically. Furthermore, the expression of B-cell translocation gene 2 （BTG2）, a putative target gene of miR-18, was analyzed in hepatocellular carcinoma tissues and the surrounding non-cancerous tissues by RT-PCR and northern blot. Results miR- 18 was over-expressed in HepG2 cells compared to L02 cells. Altogether 609 genes, including genes involved in cell proliferation, differentiation, apoptosis and tran- scriptional regulation, are identified as putative miR-18 targets. The mRNA level of BTG2 was much lower in hepatocellular carcinoma tissues than in the corresponding non-cancerous tissues. Conclusion miR-18 is over-expressed in HepG2 cells compared to L02 cells, and it may negatively regulate the expression of BTG2, a tumor suppressor gene.

关 键 词：癌 肝细胞 基因表达调控 肿瘤 细胞增殖 微RNA 18 B细胞易位基因2 

分 类 号：R735.7[医药卫生—肿瘤] R975.5[医药卫生—临床医学]