微囊藻毒素与饮用水消毒副产物复合诱导草鱼淋巴细胞凋亡  

Apoptosis of grass carp(Ctenopharngodon idellus) lymphocytes induced by the combination of microcystins and disinfection by-products in drinking water

作　　者：倪婉敏[1] 张杭君[1,2] 张建英[1] 姚超英[3] 

机构地区：[1]浙江大学环境与资源学院生态环境研究中心,杭州310028 [2]杭州师范大学生命与环境科学学院,杭州310036 [3]杭州市职业技术学院,杭州310004

出　　处：《应用生态学报》2009年第1期228-232,共5页Chinese Journal of Applied Ecology

基　　金：浙江省科技厅科技攻关项目(2007C23040);浙江省教育厅项目(20071257);浙江省“新苗人才计划”资助项目(2007G60G2010047)

摘　　要：针对饮用水中消毒副产物与微囊藻毒素的低剂量复合污染问题,采用离体细胞培养诱导方法,以草鱼淋巴细胞为暴露受体,研究了MCLR、MCRR两种微囊藻毒素和CHClBr2、CHCl2Br两种消毒副产物的单独与联合毒效应.结果表明:4种污染物在试验浓度下暴露2h即能使草鱼淋巴细胞发生凋亡,且呈显著的剂量-效应关系;1nmol·L-1MCLR、MCRR分别和1～100nmol·L-1的CHCl2Br、CHClBr2的联合作用均表现为相加作用,并且均呈显著的剂量-效应关系.草鱼淋巴细胞凋亡率可作为一种有效的指标来评价微囊藻毒素及消毒副产物复合污染引起的细胞毒性.Aiming at the combined pollution of low dose microcystins （MCs） and disinfection byproducts （DBPs） in drinking water, the individual and combined cytotoxic effects of two MCs （MCLR and MCRR） and two DBP compounds （CHClBr2 and CHCl2Br） on the apoptosis of isolated grass carp （Ctenopharngodon idellus） lymphoeytes were examined by using in vitro bioassays. The results showed that after 2 hours exposure to the four pollutants within the range of test concentrations, the apoptosis of isolated lymphocyte occurred, and there existed significant dose-effect relationship. The combined cytotoxic effects of 1 nmol · L^-1 MCLR or MCRR and 1-100 nmol · L^-1 CHC1lBr2 or CHCl2Br were all of additive, and the apoptosis percent was highly correlated with the exposure dose. It was suggested that the apoptosis percent of grass carp lymphocytes could be used as an effective indicator to evaluate the cytotoxicity caused by the combined pollution of MCs and DBPs.

关 键 词：微囊藻毒素 细胞凋亡 消毒副产物 复合污染 细胞毒性 

分 类 号：X174[环境科学与工程—环境科学]