水牛伊氏锥虫人工感染抗体规律及其广西地区自然感染状况的初步调查  

A preliminary investigation of Trypanosoma evansi infection in Guangxi water buffaloes

作　　者：何木荣[1] 施开创 陈汉忠[1] 杨兴胜 白波[1] 王柏生[1] 

机构地区：[1]广西大学动物科技学院,广西南宁530005 [2]广西区动物疫病预防控制中心,广西南宁530001 [3]广西钦州市动物卫生监督所,广西钦州530008

出　　处：《中国预防兽医学报》2009年第1期33-36,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金项目(30660140);广西教育厅科研项目(200620)

摘　　要：通过实验感染,研究伊氏锥虫在水牛体内抗体及虫体消长的规律。ELISA检测结果表明,水牛感染伊氏锥虫后第6d检出抗体,而且在2个多月的监测时间里,抗体都维持在较高水平(OD450nm>0.3),因此可利用ELISA方法对水牛感染伊氏锥虫进行早期诊断和监测;水牛感染伊氏锥虫后,即出现一个2～3周左右的虫血症峰期,然后虫血症峰期迅速下降,在虫血症峰期下降后1个多月的监测时间里,虫血症维持在一个很低的水平(≤1条虫体/视野),水牛感染伊氏锥虫后这种峰期短无明显症状、虫血症水平低的现象,给临床诊断和血液镜检虫体造成了一定的困难。对广西部分地区79份血清样品水牛检测的结果表明,伊氏锥虫抗体阳性率达到29.1%,说明广西各地水牛感染伊氏锥虫的现象普遍存在,而且感染率比较高,为了保证本地区水牛产业的健康发展,有必要对水牛伊氏锥虫感染进行有效的监测。The antibody dynamics and parasitemia of Trypanosoma evansi in experimentally infected buffaloes were studied. Antibodies in the blood could be detected six day after infection and then maintained at a comparatively high level （OD450nm〉0.3）. This result indicated that a early diagnoses and routine inspection by ELISA for T.evansi infection in buffaloes is possible. A peak period about 2 to 3 weeks of parasitemia was observed after infection, and then drop sharply to a very low-level. A preliminary investigation was conducted by ELISA on 79 serum samples from part region of Guangxi and 23 serum samples were detected positive （29.1%）. These results proved that T.evansi infection of buffaloes is prevalent in the region of Guangxi.

关 键 词：伊氏锥虫 抗体 水牛 感染 

分 类 号：S858.23[农业科学—临床兽医学]