BEL7402细胞低剂量辐射后细胞周期和相关蛋白表达的变化  

Effect of low dose radiation on expression of cell cycle and related proteins of BEL7402 cells

作　　者：许颖[1] 马克威[1] 李薇[1] 王冠军[1] 

机构地区：[1]吉林大学第一医院血液肿瘤科,吉林长春130021

出　　处：《中国老年学杂志》2009年第2期181-184,共4页Chinese Journal of Gerontology

基　　金：国家自然基金资助(No.30370439);教育部高等学校博士学科点专项科研基金资助(No.20020183015)

摘　　要：目的研究低剂量辐射(LDR)对人肝癌细胞(BEL7402)增殖、细胞周期及相关信号蛋白表达的影响,为LDR临床应用提供理论依据。方法体外培养BEL7402,分为7个实验组:假辐射组(0 mGy)、5个LDR组(25,50,75,100,200 mGy)及高剂量辐射组(1 Gy)。辐射后用细胞计数法及噻唑蓝比色试验(MTT)检测细胞增殖率,用流式细胞术测定细胞周期中G0/G1,S,G2/M期的比例。应用蛋白分析系统分析检测细胞周期及相关信号蛋白共40个蛋白的表达。结果细胞计数及MTT试验,5个LDR组BEL7402细胞增殖率与假辐射组比较差异不显著(P>0.05)。流式细胞术试验,5个LDR组BEL7402细胞12、24 h G0/G1期细胞比例增高,但与假辐射组比较差异不显著(P>0.05);S期比例降低,与假辐射组比较差异显著(P<0.05);G2/M期比例升高,与假辐射组比较差异显著(P<0.05)。48 h后G0/G1,S,G2/M期与假辐射组比较差异不显著(P>0.05)。蛋白分析试验LDR后8种蛋白表达降低。结论LDR对BEL7402细胞增殖无促进作用,但在一定程度抑制增殖及细胞周期相关蛋白的表达。Objective To study the effects of low dose radiation（LDR） on expression of cell cycle and related proteins of BELT402 cell. Methods Human hepatoma cell line （BELT402） were divided into 0 （sham radiation group）, 25,50,75,100,200 mGy （LDR subgroups） and 1 Gy （high dose radiation group）. Cell proliferation rate was detected by cytometry and MTT method. G0/G1, S, G2/M of cell cycles were determined by flow cytometry. The cell cycle and the related forty signal proteins expressions were analyzed by Protein Array system. Results The BEL7402 cell proliferation rate of five LDR subgroups had no difference from that of sham irridation group in cell counts and MTT assay results. In flow eytometry assay, G0/G1 cell ratio increased at 12 and 24 h in five LDR subgroups, but had no difference from that of sham irridiation group ; but S phase cell ratio decreased, G2/M retion increased, had significant difference from those of sham irridiation group （P 〈 0. 05 ）. There was no significant difference in G0/G1, S, G2/M between five LDR subgroups and sham irridiation group. Conclu- sions LDR has no stimulating effect on BELT402 cell. However, to some extent LDR could suppress the expression of some proteins related to proliferation and cell cycle.

关 键 词：低剂量辐射 肝癌(BEL7402) 信号 

分 类 号：R735.7[医药卫生—肿瘤]