混菌同步发酵三七糠关键参数的测定  

STUDIES ON KEY PARAMETE OF MIXED FUNGI CO FERMENTING IN HUSK

作　　者：殷月兰[1] 王永坤[1] 朱国强[1] 周继宏[1] 

机构地区：[1]江苏农学院动物医学系

出　　处：《江苏农学院学报》1998年第2期9-12,共4页Jiangsu Agricultural Research

基　　金：江苏省"九五"农业科技攻关重点项目

摘　　要：绿色木霉（Ｙ９６３０１）、米曲霉（Ｌ９６２０１）、白地霉（Ｙ９６１０１）、产朊假丝酵母（Ｐ９６４０１）的生物学特性研究表明，Ｙ９６３０１在产酶培养基上具有较高的纤维素酶（ＣＭＣａｓｅ、ＦＰａｓｅ、βＧａｓｅ）活性，其中ＣＭＣ酶活力可达到４５１１ＩＵ，ＦＰ酶活力可达到１０４９ＩＵ，纤维素降解率为２６．９％。在三七糠培养基进行混菌发酵试验表明，Ｌ９６２０１、Ｙ９６１０１、Ｐ９６４０１是Ｙ９６３０１的最佳配伍菌株。在３０℃、ｐＨ６的条件下，培养２８ｈ可使产品纯蛋白含量达到２２．１３％，纤维素降解率为３０．７％。该发酵产品经喂猪初步试用，适口性好，可部分替代动物蛋白和植物蛋白。The biological characteristics of four strains fungi, named Trichoderma koningii (Y96301), Asperigillus oryzae (L96201), Geotrichum candidum link (Y96101) and Candidum utilis (P96401), were studied. Y96301 had high level cellulose enzyme (including CMCase, FPase and βGase)activty, its enzyme activities of CMC and FP reached 4511 IU and 1049 IU respectively. The experiments in fermentation on husk culture by mixed fungi showed that L96201, Y96101 and P96401 could best match Y96301. With 28 h culture at 30℃ under pH6 condition, the final product contained 22 13% pure protein, the degradation rate of cellulose was 30 7%. The product had applied in feeding pigs, the experiment result showed that the product could stimulate appetite and partly substitute plant protein and animal protein.

关 键 词：真菌 参数 纤维素酶 混合培养 三七糠 

分 类 号：S816.6[农业科学—饲料科学]