DNA末端标记法检测番荔枝内酯诱导人乳癌细胞凋亡  

STUDY ON ACETOGENININDUCED APOPTOSIS IN HUMAN BREAST CANCER CELL LINE BY TUNEL ASSAY

作　　者：程冠军[1] 潘启超 朱振宇[1] 周宁宁[1] 冯公侃[1] 杨小平[1] 

机构地区：[1]中山医科大学

出　　处：《中山医科大学学报》1998年第2期109-111,共3页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：国家教委博士点基金

摘　　要：目的：用ＤＮＡ末端标记法（ＴＵＮＥＬ法）研究番荔枝内酯凋亡诱导作用，以及ＴＵＮＥＬ法用于凋亡检测和抗癌药所致凋亡的探讨。方法：用台盼蓝拒染法，ＴＵＮＥＬ法，ＤＮＡ凝胶电泳，电镜等技术，研究番荔枝内酯对具有多药抗药性的耐阿霉素的人乳癌ＭＣＦ７／ＡＤＲ细胞的生长抑制作用及凋亡诱导。结果：电镜下观察到细胞染色质浓缩，聚集核膜下，核碎裂等典型的凋亡细胞形态学改变。凝胶电泳可见明显的ＤＮＡ梯形条带。ＴＵＮＥＬ法检测的凋亡细胞明显多于形态学改变的凋亡细胞。结论：番荔枝内酯对人乳癌ＭＣＦ７／ＡＤＲ细胞具有明显生长抑制作用，半数抑制浓度（ＩＣ５０）为１０．５ｍｇ／Ｌ，并诱导其凋亡。ＴＵＮＥＬ法可配合其他方法用于检测抗癌药诱导的人癌细胞凋亡及抗癌药的评价。Objective:To investigate the induction of apoptosis of human breast cancer cell line MCF7/ADR by acetogenins, to dectect apoptosis by TUNEL assay and to evaluate its significance in new anticancer drug. Methods:TUNEL assay, DNA gel electrophoresis, electron microscopy were performed to detect the induction of apoptosis of MCF7/ADR cell line by acetogenins. Results:Condensation of chromatin clinged to periphery of the nucleus and fragmentation were detected by elctron microscopy, DNA ladders were observed in gel electrophoresis. Number of apoptotic cells labelled by TUNEL assay were more than that of seen with morphology changed. Conclusion:acetogenins can induce apoptosis of MCF7/ADR cell line, TUNEL assay can be used to detect apoptosis induced by anticancer drug and to evaluate new anticancer drug.

关 键 词：番荔枝内酯 乳腺肿瘤 DNA末端标记 细胞凋亡 

分 类 号：R737.905[医药卫生—肿瘤]