纤维粘连蛋白的导入对BCG与膀胱癌细胞粘附的影响  

Influence of Fibronectin gene transfection on BCG adherence to bladder cancer cells

作　　者：张文颖[1,2,3] 徐勇[1,2,3] 马腾骧[1,2,3] 高雅[1,2,3] 

机构地区：[1]天津市泌尿外科研究所 [2]北京国防科工委总医院 [3]天津医科大学第二医院泌尿外科

出　　处：《中华泌尿外科杂志》1998年第5期293-296,共4页Chinese Journal of Urology

基　　金：国家教委优秀年轻教师基金

摘　　要：为探讨纤维粘连蛋白（Ｆｉｂｒｏｎｅｃｔｉｎ，ＦＮ）介导ＢＣＧ抗膀胱肿瘤的机制，利用转基因技术将ＦＮｃＤＮＡ导入低表达ＦＮ的膀胱癌细胞系２５３Ｊ，观察转染前后［３Ｈ］Ｔｈｙｍｉｄｉｎｅ标记的ＢＣＧ与肿瘤细胞粘附的变化。结果显示：转染后的肿瘤细胞ＦＮ表达水平明显增高；同转染前的肿瘤细胞相比可结合更多的ＢＣＧ（Ｐ＜０．００１）。提示ＦＮ的表达在ＢＣＧ与膀胱肿瘤的粘附中起着重要的作用。The changes of adhesiveness of BCG to a transitional carcinoma cell line 253J with low FN expression were investigated before and after FN cDNA transfection by lipofectinmin. The result showed that FN level was increased in the cells with more BCG adhered to. It suggested that FN expression plays a very important role in mediating BCG adherence to tumor cells.

关 键 词：膀胱肿瘤 纤维粘连蛋白 细胞粘附 

分 类 号：R737.140.2[医药卫生—肿瘤]