秋水仙碱抑制G422胶质瘤细胞生长的实验研究  

Experimental study on the restraining growth by the colchicine to the G422 glioma cell.

作　　者：周勇[1] 余化霖[1] 马以骝[1] 周厚俊[1] 田冰锋[1] 于晓[1] 

机构地区：[1]昆明医学院第一附属医院微创神经外科,云南昆明650032

出　　处：《云南医药》2008年第6期526-527,共2页Medicine and Pharmacy of Yunnan

摘　　要：目的探讨运用MTT法测定秋水仙碱在体外是否具有抑制G422胶质瘤细胞生长的作用。方法采用MTT法测定经小鼠体内传代的G422胶质瘤细胞标本对6种不同浓度秋水仙碱的体外敏感性。结果不同浓度的秋水仙碱对G422胶质瘤细胞株的生长均有一定的抑制作用,瘤细胞生长抑制率随着药物浓度增大而呈增高的趋势。结论秋水仙碱在体外具有抑制G422胶质瘤细胞生长的作用,为下一步体内实验提供了理论依据。Objective To evaluate the sensitivity of colchicine to the G422 glioma cell in vitro with colorimetric MTT assay. Methods In vitro, sensitivities of 6 different concentrations of colchicine were determined by MTT method in G422 glioma which spread generation in the mice body. The G422 glioma cells were plated in 96-well tissue culture plates and incubated for 48h with colchicine. Results The growth of the G422 glioma cell can be restrained by 6 different concentrations of colchicine. The rate of restraining growth of tumour cell increased with the concentrations of medicine. Conclusion The growth of the G422 glioma cell can be restrained by colchicine.

关 键 词：胶质瘤 秋水仙碱 化疗 

分 类 号：R739.91[医药卫生—肿瘤]