比色法测定鱼腥草注射液中聚山梨酯80含量  

Determination of Polysorbate 80 in Yuxingcao Injection by Colorimetry

作　　者：刘燕梅[1] 蒲旭峰[2] 张良[2] 雷蕾[1] 彭衡阳[1] 

机构地区：[1]成都中医药大学,成都610072 [2]成都市食品药品检测中心,成都610045

出　　处：《中国药品标准》2008年第6期434-437,共4页Drug Standards of China

摘　　要：目的:建立比色法测定鱼腥草注射液中聚山梨酯80的含量,以控制其质量及安全性。方法:依据聚山梨酯80中的聚乙氧基和铵钴硫氰酸盐反应形成蓝色复合物,可溶于二氯甲烷,用比色法测定聚山梨酯80含量。结果:聚山梨酯80在1.2129～6.0646mg内与吸光度呈良好的线性关系,其线性方程为A=0.1647C+0.0011,r=0.9998,回收率为100.79%,RSD为1.38%(n=9)。结论:该方法简单易行,准确稳定,专属性强,可用于鱼腥草注射液的质量控制。Objective： A UV method was established for the determination of polysorbate 80 in Yuxingcao Injection to control its quality and safety. Methods：To determinate Yuxingcao Injection by Colorimetry based on the blue complex which can dissolve in dichloromethane. The blue complex is the reaction product of poly ethoxy of polysorbate 80 and ammonium thiocyanate Cobalt. Results： The linear range was 1. 212 9 -6. 064 6 mg and the correlation coefficient was 0. 999 8. The average recovery was 100. 79% and RSD was 1. 38% （n = 9）. Conclusion： The method is simple, accurate, stability and exclusive, and suitable for the determination of polysor- bate 80 in Yuxingcao Injection.

关 键 词：聚山梨酯80 鱼腥草注射液 比色法 含量测定 

分 类 号：R971.1[医药卫生—药品] R259.631[医药卫生—药学]