Effects of soy isoflavone extracts on the growth of estrogen-dependent human breast cancer (MCF-7) tumors implanted in ovariectomized nude mice  

作　　者：WU Qian JIN NianZu YU Jing ZHAO RenChen YU ZePing QIAO ShanLei LU XiaoHe ZHANG ChunWen 

机构地区：[1]Department of Hygienic Analysis and Detection, School of Public Health, Nanjing Medical University, Nanjing 210029 [2]Research Institute of General Surgery, Nanjing General Hospital of PLA, Nanjing Military Command, Nanjing 210002 [3]Department of Pharmacy, Nanjing General Hospital of PLA, Nanjing Military Command, Nanjing 210002

出　　处：《Chinese Science Bulletin》2009年第1期72-77,共6页

基　　金：Supported by National Natural Science Foundation of China (Grant No.30572133)

摘　　要：In this study,we explored the effects of soy isoflavone extracts on the growth of estrogen-dependent human breast cancer (MCF-7) tumors implanted in ovariectomized athymic mice. The ovariectomized athymic mice were implanted with MCF-7 cells. They were fed with low,moderate and high doses of soy isoflavone extracts,at dietary concentrations of 6.25,12.5 and 25 g/kg,respectively. The expression of ki-67 was detected by immunohistochemistry. The pS2 expression in tumors was analyzed by real-time PCR. Estrogen level in the serum was measured by chemiluminescence enzyme immunoassay. Com-pared with the control group,dietary soy isoflavone extracts had a significant stimulatory effect on MCF-7 tumor growth in mice (P < 0.05). The ki-67 and pS2 mRNA expressions in tumors were signifi-cantly increased by 6.25 and 12.5 g/kg dose of soy isoflavone extracts (P < 0.05). And,estrogen level in serum of 6.25 and 12.5 g/kg dose groups was higher than that of control group (P > 0.05). In conclusion,in the tested dietary concentration range soy isoflavone extracts had a stimulatory effect on tumor growth. 6.25 and 12.5 g/kg doses of soy isoflavone extracts can increase the cell proliferation in tumors and induce estrogen-responsive pS2 expression.In this study, we explored the effects of soy isoflavone extracts on the growth of estrogen-dependent human breast cancer （MCF-7） tumors implanted in ovariectomized athymic mice. The ovariectomized athymic mice were implanted with MCF-7 cells. They were fed with low, moderate and high doses of soy isoflavone extracts, at dietary concentrations of 6.25, 12.5 and 25 g/kg, respectively. The expression of ki-67 was detected by immunohistochemistry. The pS2 expression in tumors was analyzed by real-time PCR. Estrogen level in the serum was measured by chemiluminescence enzyme immunoassay. Compared with the control group, dietary soy isoflavone extracts had a significant stimulatory effect on MCF-7 tumor growth in mice （P 〈 0.05）. The ki-67 and pS2 mRNA expressions in tumors were significantly increased by 6.25 and 12.5 g/kg dose of soy isoflavone extracts （P 〈 0.05）. And, estrogen level in serum of 6.25 and 12.5 g/kg dose groups was higher than that of control group （P〉 0.05）. In conclusion, in the tested dietary concentration range soy isoflavone extracts had a stimulatory effect on tumor growth. 6.25 and 12.5 g/kg doses of soy isoflavone extracts can increase the cell proliferation in tumors and induce estrogen-responsive pS2 expression.

关 键 词：大豆异黄酮提取物 乳腺癌 裸鼠 MCF-7 雌激素 

分 类 号：R737.9[医药卫生—肿瘤]