薤中抗凝和抗癌活性成分的结构鉴定  

STRUCTURAL ELUCIDATION OF THE ANTICOAGULATION AND ANTICANCER CONSTITUENTS FROM ALLIUM CHINENSE

作　　者：姜勇[1,2] 王乃利[1,2] 姚新生[1,2] 北中进[1,2] 

机构地区：[1]沈阳药科大学天然药物化学教研室 [2]日本大学药学部生药教研室

出　　处：《药学学报》1998年第5期355-361,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金

摘　　要：从百合科葱属植物薤（Ａｌｉｕｍｃｈｉｎｅｎｓｅ）鳞茎的抗凝和抗癌活性部位中，分离得到了６个化合物。经过化学方法和光谱分析（ＩＲ，ＥＩＭＳ，１ＨＮＭＲ，１３ＣＮＭＲ，１Ｈ１ＨＣＯＳＹ，ＨＭＢＣ，ＨＭＱＣ和ＮＯＥＳＹ谱），鉴定它们的结构分别为（２５Ｒ，Ｓ）５αｓｐｉｒｏｓｔａｎｅ３βｏｌ３Ｏ｛βＤｇｌｕｃｏｐｙｒａｎｏｓｙｌ（１→２）［βＤｇｌｕｃｏｐｙｒａｎｏｓｙｌ（１→３）］βＤｇｌｕｃｏｐｙｒａｎｏｓｙｌ（１→４）βＤｇａｌａｃｔｏｐｙｒａｎｏｓｉｄｅ｝（１），（２５Ｒ，Ｓ）５αｓｐｉｒｏｓｔａｎｅ３βｏｌ３Ｏ｛βＤｇｌｕｃｏｐｙｒａｎｏｓｙｌ（１→２）［βＤｇｌｕｃｏｐｙｒａｎｏｓｙｌ（１→３）］（６ａｃｅｔｙｌβＤｇｌｕｃｏｐｙｒａｎｏｓｙｌ）（１→４）βＤｇａｌａｃｔｏｐｙｒａｎｏｓｉｄｅ｝（２），（２５Ｒ，Ｓ）５αｓｐｉｒｏｓｔａｎｅ２α，３βｄｉｏｌ３Ｏ｛βＤｇｌｕｃｏｐｙｒａｎｏｓｙｌ（１→２）ＯβＤｇｌｕｃｏｐｙｒａｎｏｓｙｌ（１→４）βＤｇａｌａｃｔｏｐｙｒａｎｏｓｉ?Six compounds were isolated from the anticoagulation and anticancer fractions of the bulbs of Allium chinense G. Don. On the basis of chemical evidence and spectral analysis (IR, EIMS, 1HNMR, 13CNMR, 1H1H COSY, HMBC, HMQC and NOESY), their structures were established as (25R,S)5αspirostane3βol 3O{βDglucopyranosyl(1→2)[βDglucopyranosyl(1→3)]βDglucopyranosyl(1→4)βDgalactopyranoside} (1), (25R,S)5αspirostane3βol 3O{βDglucopyranosyl (1→2)[βDglucopyranosyl(1→3)](6acetylβDglucopyranosyl)(1→4)βDgalactopyranoside} (2), (25R,S)5αspirostane2α,3βdiol 3O{βDglucopyranosyl(1→2)OβDglucopyranosyl(1→4)βDgalactopyranoside} (3), (25S)24OβDglucopyranosyl3β,24βdihydroxy5αspirost3Oαarabinopyranosyl(1→6)βDglucopyranoside (4), chinenoside II (5) and 2,3,4,9tetrahydro1methyl1Hpyrido indole3carboxylic acid (6). 4 is a new steroidal saponin, named chinenoside VI. Compounds 1 to 3 are three pairs of steroidal saponin epimers. Among them, the 25S epimer of 2 is first reported, the 25R epimer of 2 and compound 6 were isolated from this title plant for the first time. The relative configuration of compound 6 was firstly determined by NOESY spectrum, and signals of C, H were assigned definitely. 

关 键 词：薤 抗凝活性 抗癌活性 甾体皂甙 差向异构体 

分 类 号：R284.1[医药卫生—中药学]