甘肃胃癌高发区人群p53基因Arg72Pro多态性的初步研究  

Study of p53 Gene Codon 72 Arg/Pro Polymorphism in High Incidence Area of Gastric Cancer in Gansu Province

作　　者：单勇[1] 李玉民[2] 李汛[2] 周文策[2] 石斌[2] 何明彦[2] 朱克祥[2] 何建宏[2] 刘涛[3] 何文婷[4] 俸铁岚[5] 

机构地区：[1]兰州大学临床医学院,兰州730000 [2]兰州大学第一医院普外二科,兰州730000 [3]兰州大学第一医院肿瘤研究所,兰州730000 [4]兰州大学第一医院肿瘤研究,兰州730000 [5]兰州大学第一医院手术室,兰州730000

出　　处：《中国普外基础与临床杂志》2009年第1期12-17,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金资助(项目编号:30870964)~~

摘　　要：目的探讨p53基因第72位密码子（Arg72Pro）多态性与胃癌高发区甘肃河西地区汉族人群胃癌遗传易感性的关系，并探讨Arg72Pro多态性与环境因素、胃癌发生部位、病理分型等的相互关系。方法采用TaqMan实时荧光定量PCR方法检测140例胃癌患者、110例胃癌癌前病变患者以及125例健康人群的外周血标本p53基因Arg72Pro的多态性，并比较其不同的基因型与胃癌风险的关系；对胃镜活检以及手术切除标本应用WarthinStarry法（W—S法）判断幽门螺杆菌（Hp）感染情况。结果胃癌组、癌前病变组及健康对照组的Pro等位基因频率分别为0．543、0．482和0．472，Pro基因型携带者的胃癌发病风险是Arg／Arg基因型携带者的1．846倍[95％CI（1．006～3．387），P=0．046；。经Logistic回归筛选确定了饮食、Hp感染、精神因素以及肿瘤家族史为胃癌的危险因素，并与Pro基因型具有加乘作用。p53基因型分布和胃癌的部位及病理分型具有相关性（P〈0．05）。结论p53Arg72Pro多态性与甘肃河西地区汉族人群胃癌遗传易感性相关，值得进一步进行功能学探讨及大样本人群验证。Objective To investigate the relationship of p53 codon 72 polymorphism and susceptibility to gastric cancer in high incidence area of Hexi area of Gansu province. Methods The Arg/Pro polymorphism of p53 gene was detected by real-time PCR in 140 patients with gastric cancer, 110 patients with gastric precancerous lesion and 125 healthy controls; Helicobacter pylori （Hp） infection was detected by Warthin-Starry silver method. Results The Pro allele frequencies of p53 gene in gastric cancer cases （0. 543） were higher than those in gastric precancerous lesion （0. 482） and controls （0. 472）. The Pro genotype had a more than 1. 846 fold increased risk of gastric cancer （OR 1.846; 95%CI （1.006-3.387）; P=0.046]. With statistical analysis, the genotype of p53 gene was correlated with location and Lauren＇s histological type （P〈0.05）. A significantly higher risk of gastric cancer was also seen in cases with p53 Pro genotype, food, Hp infection, positive mind factor and positive family history. Conclusion There is a strong correlation between the p53 gene codon 72 Arg/Pro polymophism and susceptibility to gastric cancer in Hexi area of Gansu province and the Pro/Pro genotype may be one of the major risk factors in patients with gastric cancer.

关 键 词：P53基因 单核苷酸多态性 易感性 胃癌 

分 类 号：R735.2[医药卫生—肿瘤] R730.231[医药卫生—临床医学]