免试剂离子色谱法测定肉制品中的多聚磷酸盐  

Determination of polyphosphates in meat by free reagent ion chromatography

作　　者：许迪明[1] 杨娟芬[1] 任飞[1] 

机构地区：[1]宁波出入境检验检疫局,浙江宁波315012

出　　处：《中国卫生检验杂志》2008年第12期2553-2554,2647,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立肉制品中的正磷酸盐、焦磷酸盐和三聚磷酸盐的分析方法。方法：用免试剂离子色谱（RFIC）带电导检测器进行分离测定,色谱柱：IonPac AS11-HC Analytical（4×250 mm）,保护柱：IonPac AG11-HC Guard（4×50 mm）,流动相为KOH水溶液,进行梯度洗脱。样品经水超声提取后,用免试剂离子色谱电导检测进行分析检测,结合保留时间和电导检测值进行定性分析。结果：该法正磷酸盐的回收率为98.8%～102.1%,精密度RSD为1.24%,以原样计方法检出限为4μg/g,焦磷酸盐的回收率为89.8%～91.5%,精密度RSD为0.65%,以原样计方法检出限为4μg/g,三聚磷酸盐的回收率为92.6%～94.4%,精密度RSD为0.82%,以原样计方法检出限为10μg/g。结论：该方法具有前处理方便、分析速度快、结果准确等优点。能够满足检测不同的肉制品中多聚磷酸盐含量的要求。Objective：To establish a method for determination of polyphosphates in meat.Methods：Polyphosphates were determined by free reagent ion chromatography equipped suppressed conductivity.Chromatographic column： IonPac AS11-HC（4×250 mm）,Mobile phase：KOH water solution,gradient elution.Polyphosphates are extracted from meat in the ultrasonic with precipitator added in deionized water.The qualification analysis is done by using retention time and conductivity.Results：The average recovery for phosphate was 98.8%～102.1%,relative standard deviations 1.24%,the limits of detection for phosphate 4 μg/g.The average recovery for pyrophosphate was 89.8%～91.5%,relative standard deviation 0.65%,the limits of detection for pyrophosphate 4 μg/g.The average recovery for trimetaphosphate was 92.6%～94.4%,relative standard deviations 0.82% and the limit of detection for trimetaphosphate 10 μg/g.Conclusion：The method is simple and fast and has a good separating effect.So it could meet the need for determination of polyphosphates in meat.

关 键 词：肉制品 多聚磷酸盐 免试剂离子色谱 

分 类 号：O657.75[理学—分析化学]