γδT细胞对肝癌细胞的杀伤作用  

Cytotoxic effects of human γδT cells on hepatocellular carcinoma cells in vitro

作　　者：贺潇[1] 马勇[1] 桂万羊 

机构地区：[1]蚌埠医学院医学检验系,安徽省蚌埠233030 [2]解放军第一二三医院肝病中心

出　　处：《中国基层医药》2008年第11期1770-1771,1937,共3页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的探讨γδT细胞对肝癌细胞的杀伤作用。方法异戊烯焦磷酸（isopentenyl pyrophosphate，IPP）和rhIL-2活化正常人外周血单个核细胞（PBMC），用流式细胞仪分析活化后的18T细胞含量，用ELISA法检测培养上清液中IFN-γ含量，用乳酸脱氢酶释放法测定活化γδT细胞的杀伤活性。结果体外活化的PBMC在10d时16T细胞比例从扩增前（4．34±1．79）％增加到（55．65±6．88）％，PBMC经IPP刺激培养后IFN-γ分泌量较不含IPP的对照组有显著增高，至第7天达峰值，活化后的淋巴细胞对肝癌细胞有较高杀伤活性。结论IPP作为刺激剂可获得大量γδT细胞，刺激PBMC后可分泌大量IFN-γ，γδT细胞在体外对肝癌细胞有较高的杀伤活性。Objective To investigate the cytotoxic effects of human γδT cells on hepatoceilular carcinoma ceils in vitro. Methods Normal human PBMC were activated by IPP and rhIL-2. The expansion of γδT cells were analyzed by flow cytometry. The concentration of interferon gamma in the supernatants was detected by ELISA. The cyto- toxicity of activated lymphocytes against hepatocellular carcinoma cells were measured by the lactate dehydrogenase release assay in vitro. Results After PBMC activated by IPP and rhIL-2 for 10 days,the proportion of γδT cells were expanded from （ 4. 34 ± 1.79 ） % to （ 55.65±6. 88 ） %. The secretion of interferon gamma expanded significantly after PBMC stimulated by IPP, and reached its peak value at the seventh day. The secretion of interferon gamma is lower if it has no IPP. The activated lymphocytes stimulated by IPP were detected to have cytotoxicity in vitro against human hepatocellular carcinoma cells. Conclusion The high proportion of γδT cells can be gained in human PBMC activated by IPP, PBMC stimulated by IPP can secret interferon gamma effectively and the activated γδT cells have high cytotoxicity in vitro against human hepatocellular carcinoma cells.

关 键 词：ΓΔT细胞 肝肿瘤 淋巴毒素 

分 类 号：R735[医药卫生—肿瘤]