重组人肝脏增生因子克隆表达纯化及生物活性的探讨  

The cloning, expression and pruification of recombinant

作　　者：曾晓波[1] 苏先狮[1] 

机构地区：[1]中南大学湘雅二医院肝病研究所,湖南省长沙410011

出　　处：《中国基层医药》2008年第11期1838-1840,1939,共4页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的构建人肝脏再生增强因子（hALR）原核表达载体，为基因工程大量制备hALR提供基础。方法构建hALR表达载体pGEX-3X-hALR，诱导表达GST—hALR，用GST Agawse柱亲和层析，再用Xa因子切除GST得到hALR蛋白；进一步用MTF法及动物实验检测hALR活性。结果成功地构建了hALR表达载体pGEX-3X-hALR，并纯化出hALR蛋白；体外细胞实验及动物实验结果与文献报道相反。Objective To provide a technique of preparation recombinant hALR by gene engineering in larger scale. Methods Construct hALR（ human augmenter of liver regeneration） protein expression vector pGEX-3X- hALR,induce the expreesion of hALR, and pruified the protein by attinity chromatography. Results Succeed in constructing the vector pGEX-3X-hALR and obtained the recombinant hALR protein. Conclusion The mothod could be used for preparation recombinant hALR by gene engineering in larger scale.

关 键 词：人肝脏增生因子 克隆 分子 蛋白纯化 活性检测 

分 类 号：R73[医药卫生—肿瘤]