超临界萃取当归挥发油及阿魏酸正交试验研究  

Study on the SFE-CO_2 Process of Extraction for Volatile Oil and Ferulic Acid from Angelica by Orthogonal Test

作　　者：刘丹[1] 刘超英[1] 韩建伟[1] 

机构地区：[1]湖北中医学院中药资源与中药复方省部共建教育部重点试验室,武汉430061

出　　处：《中国药师》2008年第12期1438-1440,共3页China Pharmacist

基　　金：湖北省教育厅科学技术研究重点项目(D20081608)

摘　　要：目的:优选超临界二氧化碳流体(SFE-CO_2)萃取当归挥发油和阿魏酸的工艺条件。方法:以阿魏酸的提取量和挥发油的得率为考察指标,以高效液相色谱法测定阿魏酸含量,采用正交试验对提取条件优选。结果:优选萃取条件是:当归粉碎为20～60目,75%的乙醇作为夹带剂,萃取压力30Mpa,萃取温度60℃。结论:优选工艺萃取的挥发油和阿魏酸提取量较高,所得浸膏量较低。Objective： To optimize the supercritical CO2 fluid extraction process for volatile oil and ferulic acid from angelica. Method ： The yield of volatile oil and ferulic acid was regarded as the markers and the content of ferulic acid was determined by HPLC. The extraction processing conditions were optimized by orthogonal test. Result： The optimized extraction technique was as follows： The size of angelica powder was between 20 - 60 mu, and angelica was extracted under the pressure of 30 Mpa at 60 ℃ and 75% EtOH as entrainer. Conclusion： The volatile oil and ferulie acid extracting by the the optimum extraction process are higher and the extract yield is lower.

关 键 词：超临界CO2萃取 正交试验 当归挥发油 阿魏酸 

分 类 号：R284.2[医药卫生—中药学]