高效液相色谱法测定子宫锭中儿茶素的含量  

Determination the Catechin in Uterus Lozenge by HPLC

作　　者：林岩[1] 杨钊[2] 吴爱英[2] 赵鲁青[2] 

机构地区：[1]滕州市中心人民医院药剂科,山东滕州277500 [2]青岛市药品检验所

出　　处：《中国药师》2008年第12期1481-1483,共3页China Pharmacist

摘　　要：目的:建立子宫锭中儿茶素的含量测定方法。方法:色谱柱为Agilent HC C_(18)柱(250mm×4.6mm,5μm),流动相为0.04mol·L^(-1)枸橼酸溶液-N,N-二甲基甲酰胺-四氢呋喃(THF)(45:8:2),检测波长280nm,柱温35℃,流速1.0ml·min^(-1)。结果:儿茶素在501.2～1754.2ng之间呈良好的线性关系。平均加样回收率为98.47%,RSD=1.92%(n=9)。结论:方法简便可靠,分离度好,可用于子宫锭的质量控制。Objective： To establish a method for determination the content of catechin in Uterus Lozenge, Method： Agilent HC C18 column （ 250 mm × 4.6 mm, 5 μm） was used,0.04 mol· L^-1 citric acid-N, N-dimthylformamide-THF （45： 8： 2 ） was used as mobile phase. The UV detector wavelangth was 285 nm, column temperature was at 35 ℃ and the flow rate was 1.0 ml· min^-1. Result： The linear range of cateehin was 501.2 - 1754.2 ng. The average recovery was 98.47% ,RSD = 1.92% （ n = 9 ）. Conclusion： The method is simple, reliable, with a good separation, so it can be used for the quality control of Uterus Lozenge.

关 键 词：子宫锭 儿茶素 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]