Screening of Extraction Methods for Glycoproteins from Jellyfish (Rhopilema esculentum) Oral-Arms by High Performance Liquid Chromatography  

作　　者：REN Guoyan LI Bafang ZHAO Xue ZHUANG Yongliang YAN Mingyan HOU Hu ZHANG Xiukun CHEN Li 

机构地区：[1]Food and Bioengineering Department, Henan University of Science and Technology, Luoyang 471003, P. R. China [2]College of Food Science and Engineering, Ocean University of China, Qingdao 266003, P. R. China

出　　处：《Journal of Ocean University of China》2009年第1期83-88,共6页中国海洋大学学报（英文版）

基　　金：support from the National High Technology Research and Development Program of China (No.2006AA09Z438)

摘　　要：In order to select an optimum extraction method for the target glycoprotein （TGP） from jellyfish （Rhopilema esculentum） oral-arms, a high performance liquid chromatography （HPLC）-assay for the determination of the TGP was developed Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear （r= 0.9984, y=4.5895x＋47.601） over concentrations ranging from 50 to 400mgL^-1. The mean extraction recovery was 97.84% （CV2.60%）. The fractions containing TGP were isolated from jellyfish （R esculentum） oral-arms by four extraction methods： 1） water extraction （WE）, 2） phosphate buffer solution （PBS） extraction （PE）, 3） ultrasound-assisted water extraction （UA-WE）, 4） ultrasound-assisted P/3S extraction （UA-PE）. The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL （7.8 mm×300 ram） column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oralarms, a high performance liquid chromatography (HPLC)–assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.

关 键 词：HPLC GLYCOPROTEIN JELLYFISH extraction method 

分 类 号：P73[天文地球—海洋科学]