抗菌肽FALL-39及其突变肽对诱生型一氧化氮产生的影响  

Effect of antibacterial peptide FALL-39 and its mutant peptides on production of nitric oxide induced by lipopolysaccharides

作　　者：郝杰[1] 杨云霞[1] 王伯瑶[1] 

机构地区：[1]四川大学华西基础医学与法医学院,成都610044

出　　处：《国际病理科学与临床杂志》2008年第6期461-464,共4页Journal of International Pathology and Clinical Medicine

基　　金：国家自然科学基金(39670683)~~

摘　　要：目的:比较人抗菌肽FALL-39及其突变肽对细菌脂多糖(lipopolysaccharides,LPS)介导的诱生型一氧化氮(nitric oxide,NO)产生的影响。方法:体外常规培养人单核吞噬细胞THP-1,LPS刺激细胞,分别用人抗菌肽FALL-39以及突变肽FALL-39-Lys24,FALL-39-Lys32,FALL-39-Lys24′32处理细胞。应用RT-PCR方法测定LPS诱生型一氧化氮合酶(induced nitric oxide synthase,iNOS)以及FALL-39 mRNA的表达,用硝酸还原酶法测定细胞培养上清液中NO的含量。小鼠腹腔注射更生霉素和LPS建立内毒素血症模型,分别用抗菌肽处理动物,取血测定血清中NO的含量。结果:LPS刺激FALL-39 mRNA的表达增加;同时也诱导NO mRNA的表达以及细胞培养上清液中NO含量的增加。人抗菌肽FALL-39及其突变肽FALL-39-Lys24,FALL-39-Lys32,FALL-39-Lys24′32可以抑制LPS诱导的NO的产生;其中FALL-39-Lys24′32的抑制作用最为明显。结论:抗菌肽FALL-39及其突变肽具有抗内毒素作用,其机制与抑制LPS诱导的NO产生有关。Objective To study the effect of antibacterial peptide FALL-39 and it mutantpetides on production of nitric oxide （NO） induced by lipopolysaccharides （LPS）. Methods THP-1 cells were treated with LPS, FALL-39 and it mutant petides FALL-39-Lys24, FALL-39-Lys32, FALL-39- Lys24＇32. Semi-quantitative reverse-transcription polymerase chain reaction （RT-PCR） was used to determine the FALL-39 and iNOS mRNA expression. NO content was detected by using nitrate reductase method. The endotoxima model in BALB/C mice was created by using LPS and actinomycin D. The mice were treated with the peptides and NO content in serum was detected. Results The FALL-39 and iNOS mRNA expression were increased induced by LPS. FALL-39 and it mutant petides inhibited the induced nitric oxide synthase （iNOS） mRNA expression and the production of NO in THP-1 cells. Among them, FALL-39-Lys24＇32 had the most potential effect. Conclusion Antibacterial peptide FALL-39 and its mutantpetides have anti-endotoxin effect, and this effect is associated with inhibiton of iNOS mRNA expression and NO production.

关 键 词：抗菌肽 FALL-39 突变 一氧化氮 

分 类 号：R346[医药卫生—基础医学]