肝细胞癌患者肝组织中JAB1和P27^(kip1)的表达变化及其临床意义  

Expression of JAB1 and P27^(kip1) in liver tissue of patients with hepatocellular carcinoma and its clinical significance

作　　者：王酉[1,2] 陆牡丹[1] 李鹏[3] 崔小鹏[3] 沈爱国[1] 

机构地区：[1]南通大学微生物与免疫学教研室 [2]无锡市妇幼保健院,江苏无锡214002 [3]南通大学附属医院普外科,江苏南通226001

出　　处：《基础医学与临床》2008年第12期1233-1238,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(30300099)(30770488);江苏省自然科学基金(BK2003035);江苏省高校自然科学研究项目(03KJB180109)(04KJB320114);江苏省科技指导性计划(BS2004526);"六大人才高峰"第二批项目资助计划;江苏省卫生科研项目(H200632);南通社会发展科技项目(No.S2007041)

摘　　要：目的研究肝细胞癌(HCC)组织中C-JUN激活域连接蛋白1(JAB1)的表达及与P27kip1(P27)间的关系,探讨JAB1与临床病理及预后的关系。方法免疫组化检测HCC及相应癌旁肝组织中JAB1和P27的表达。Western blot及免疫沉淀检测JAB1和P27的表达及相互作用。结果HCC中,JAB1的表达高于癌旁肝组织,P27表达低于癌旁组织。JAB1的表达与组织分化程度、甲胎蛋白(AFP)值及转移相关(P<0.05)。HCC中JAB1与P27的表达呈负相关(r=-0.7077,P<0.001),并且能免疫共沉淀。结论JAB1的表达与P27负相关,JAB1作为P27的负性调控因子,可能与HCC的恶性进展及预后相关。Objective To investigate the expression of C-JUN activation domain binding protein 1 （JAB1） and its relationship with expression of P27 protein in human hepatocellular carcinoma （HCC）, and to determine whether JAB1 is associated with clinicopathological parameters and prognosis of HCC. Methods Immunohistochemical analysis was performed to investigate the expression of JAB1 and P27 in 76 cases of HCC and adjacent nontumomus tissues. Fresh tumor tissues and their adjacent nontumorous tissues from 8 cases of HCC were collected for Western blot and immu- noprecipitation assays. Results The expression of JAB1 in HCC was significantly higher than that in adjacent nontu- morous tissues. In contrast, P27 level was higher in nontumorous liver tissues than that in HCC. JAB1 overexpression was correlated with histological differentiation, serum alpha-fetoprotein（AFP） level and metastasis （P 〈0. 05）. In addition, a negative correlation between JAB1 and P27 expression was found in HCC （r = -0. 7077, P 〈 0. 001 ）, and co-immunoprecipitation experiments demonstrated a direct interaction between JAB 1 and P 2 7 in HCC . In both Kaplan-Meier Plot and multivariate Cox regression analysis, the overexpression of JAB1 was significantly associated with poor prognosis （P 〈 0. 05 ）. Conclusion JAB1 expression is negcetively correlated with P27 expression, and JAB1, as a negative regulator of P27, may be associated with the progression and prognosis of HCC.

关 键 词：肝细胞癌 预后 JAB1 P27 

分 类 号：R735.7[医药卫生—肿瘤]