BPI_(23)-haFGF融合蛋白的鉴定及其生物活性研究  

Identification of BPI_(23)-haFGF fusion protein and its biological activities

作　　者：马艳[1] 朱家勇[1] 金小宝[1] 刘雷山[1] 王艳[1] 李娟兰[1] 

机构地区：[1]广东药学院基础学院病原生物学研究所,广东广州510006

出　　处：《基础医学与临床》2008年第12期1266-1270,共5页Basic and Clinical Medicine

基　　金：广东省自然科学基金重点项目(04105449)

摘　　要：目的研究BPI23-haFGF融合蛋白的生物学活性。方法将已构建的酵母重组表达菌株pPICZαA-BPI23-haF-GF/X-33进行甲醇诱导表达,通过亲和层析纯化和冻干浓缩后,观察融合蛋白BPI23-haFGF对细菌、NIH3T3细胞以及小鼠烫伤模型的作用。结果获得纯度约90%的融合蛋白BPI23-haFGF,BPI23-haFGF具有杀菌及促增殖的双重功能,BPI23-haFGF促增殖率与HaFGF标准品相当,当其浓度为0.40g/L时,促NIH3T3细胞增殖率最高(88.7%);在小鼠烫伤模型中,用药组比阴性对照组提前2～3d愈合。结论融合蛋白BPI23-haFGF能够改善小鼠烫伤创面的愈合,为其在临床上的应用奠定了基础。Objective To study the biological activity of BPI23-haFGF fusion protein. Methods Recombinant expression of yeast strains pPICZαA-BPI23-haFGF/X-33 was induced by methanol. After purification by affinity chromatography and concentration by lyophilization, the biological functions of fusion protein BPI23-haFGF with bacteria, NIH3T3 cells and mice scalded model were analyzed. Results The purity of fusion protein BPI23-haFGF is about 90%, it has the dual function at bactericidal and mitogenetic, and the mitogenic effects of BPI23-haFGF is considerable to Standard HaFGF. It is highest（88.7% ） when its concentration is 0. 40 mg/mL. On the mice scalded model, the BPI23-haFGF group can advance healing than the negative sample group by 2 to 3 d. Conclusion The fusion protein BPI23-haFGF can improve the healing of burn wounds, it laid the foundation for its clinical application.

关 键 词：融合蛋白BPI23-haFGF 杀菌 促增殖 烫伤愈合 

分 类 号：R96[医药卫生—药理学]