基于线粒体Cyt b基因部分序列的中国东方蜜蜂不同地理种群的系统发育  

Phylogeny of different geographic populations Apis cerana in China based on mtDNA cytochrome b gene sequences

作　　者：高鹏飞[1] 赵慧婷[1] 张春香[1] 姜玉锁[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《动物学报》2008年第6期1005-1013,共9页ACTA ZOOLOGICA SINICA

基　　金：山西省留办基金项目(No.2003051);山西省教育厅开发项目(No.200337)资助~~

摘　　要：从线粒体DNA水平上对中国境内东方蜜蜂不同地理种群的系统发育进行研究,为保护和合理利用这一宝贵的蜂种资源提供理论基础。对我国10个省市的21群东方蜜蜂mtDNA Cyt b基因片段进行了扩增、测序,并以意大利蜂、卡尼鄂拉蜂、印尼蜂作为外群进行序列分析。采用Minimum-Spanning Network方法构建系统进化树。结果显示:扩增片段长度429bp;在得到的21条同源序列中,共检测出13个变异位点,其中转换数为9,颠换数为4,并且变异位点大都发生在密码子的第三位,无碱基插入或缺失;聚类分析结果显示西方蜜蜂、印尼蜂和东方蜜蜂各为独立的蜂种;在中国东方蜜蜂群体中,吉林、海南和云南的东方蜜蜂各为一个独立的类群,其它地区的东方蜜蜂为一个类群。In order to provide the basic datum to protect and rationally develop the Apis cerana resources in China, phylogeny of different geographic populations Apis cerana in China was studied based on mtDNA Cytocbrome b gene sequences. In this study, the partial sequences of mtDNA Cytoehrome b gene of 21 colonies of Apis cerana collected from 10 provinces and the homologous sequences of Apis nigrocincta and Apis mellifera downloaded from GenBank were analyzed. Molecular phylogenetie trees were reconstructed by minimum-spanning network methods. The multiple sequence alignment was performed using special software. The results indicated that the length of PCR products was 429 bp. Among the 21 analyzed sequences there were 13 variable sites, of which 9 sites were transitions, 4 were transversions and there were no deletions or insertions in the sequences. Phylogenetic analysis based on the mtDNA Cyt b gene of 21 colonies of Apis cerana and the homologous sequences of outgroups indicated that the groups of Apis nigrocincta, Apis mellifera and Apis cerana were clustered respectively. Jilin, Hainan and Yunnan groups of Apis cerana separated from the others in China [Acta Zoologica Sinica 54 （6） ： 1005 - 1013, 2008].

关 键 词：蜜蜂 东方蜜蜂 遗传多样性 MTDNA 细胞色素B Minimum-Spanning Network 

分 类 号：Q96[生物学—昆虫学]