南方根结线虫侵染对茄子砧木幼苗根系活性氧代谢及相关酶活性的影响  

Effects of Infection with Meloidogyne incognita on Reactive Oxygen Metabolism and Correlated Enzyme Activities in Roots of Eggplant Rootstock Seedlings

作　　者：徐小明[1] 于芹[1] 徐坤[1] 董灿兴[1] 王玉光[1] 

机构地区：[1]山东农业大学园艺科学与工程学院作物生物学国家重点实验室,山东泰安271018

出　　处：《园艺学报》2008年第12期1767-1772,共6页Acta Horticulturae Sinica

基　　金：山东省自然科学基金项目(2005ZRB01001)

摘　　要：以对南方根结线虫抗性水平显著不同的两个茄子砧木为试材,研究了接种南方根结线虫对幼苗根系活性氧代谢及相关酶活性的影响。结果表明,抗病品种托鲁巴姆幼苗根系超氧阴离子(O2.-)生成速率、过氧化氢(H2O2)含量及超氧化物岐化酶(SOD)、过氧化氢酶(CAT)、几丁质酶(CHT)和β-1,3-葡聚糖酶(GLU)活性显著高于易感品种赤茄,而过氧化物酶(POD)活性则低于赤茄。南方根结线虫初次侵染前期,两个砧木幼苗根系O.2-生成速率、H2O2含量及CHT和GLU活性均迅速升高,托鲁巴姆升幅显著高于赤茄,初次侵染后期均降低;二次侵染前期又升高,但升幅较初次减小。幼苗根系SOD、CAT活性在南方根结线虫侵染前期较未接种的对照显著降低,且托鲁巴姆降幅显著大于赤茄,侵染后期则均有所增强;而POD活性变化则相反。南方根结线虫侵染还导致根系丙二醛(MDA)含量持续增加,但以赤茄较高,表明南方根结线虫侵染加剧了根系,特别是易感品种根系的膜脂过氧化程度。The effects of nematode infection on reactive oxygen metabolism and the activities of related enzymes were investigated and compared between the seedling roots of nematode-resistant eggplant variety Solanum torvum and susceptible Solanum intergriflium in this study. The results showed that the production rate of superoxide anion radical （ O2^-）, the content of hydrogen peroxide （ H2 O2 ） as well as the activities of superoxide dismutase （SOD）, catalase （CAT）, chitinase （CHT） and β-1, 3-glucanase （GLU） were rather higher in the resistant variety than susceptible one, while peroxidase （POD） activity acted contrarily in resistant and susceptible varieties. At the early stage of first infection, O2^- production rate, H2O2 content and the activities of CHT and GLU were increased rapidly in both varieties with the increasing rate higher in S. torvum than S. intergriflium. At the late stage of first infection, however, all above four indexes decreased. Interestingly, those indexes enhanced again at the late stage of second infection with a smaller increment than the first infection, especially in susceptible variety S. intergriflium. In addition, the activities of SOD and CAT in roots infected with Meloidogyne incognita was remarkably decreased with the decreasing rate higher in S. torvum than S. intergriflium at the early stage of infection circle, while their activities were increased in both varieties at the later stage of infection circle. The changes of POD activity were contrary to that of SOD and CAT during the whole infection circle. Infection by M. incognita also led to a persistent increase in malondialdehyde content in roots with S. intergriflium being higher than S. torvum, suggesting that nematode infection aggravated the extent of membrane lipid peroxidation in roots, especially in susceptible variety.

关 键 词：茄子 砧木 南方根结线虫 活性氧代谢 几丁质酶 β-1  3-葡聚糖酶 

分 类 号：S641.1[农业科学—蔬菜学]