血管内皮生长因子165基因对人胃癌细胞体外侵袭转移的影响  

Effect of vascular endothelial growth factor 165 on the invasion and metastasis of human gastric cancer cells in vitro

作　　者：乐红琴[1] 欧希龙[1] 杭程[1] 陈慧娟[1] 关云艳[1] 孙为豪[2] 

机构地区：[1]东南大学附属中大医院消化内科,江苏省南京市210009 [2]南京医科大学第一附属医院老年消化科,江苏省南京市210029

出　　处：《世界华人消化杂志》2008年第36期4036-4040,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨血管内皮生长因子165基因(vascular endothelial growth factor 165,VEGF165)对胃癌侵袭转移的影响及可能的机制.方法:通过体外培养人胃癌细胞BGC-823,分别转染Ad-GFP及Ad-VEGF165.应用Millicell小室分析VEGF165转染前后胃癌细胞迁移能力的变化;逆转录聚合酶链反应(RT-PCR)研究VEGF165转染前后BGC-823细胞MMP-2、u-PAmRNA表达变化.结果:迁移实验结果显示Ad-VEGF165组胃癌细胞迁移能力高于Ad-GFP组及对照组(217.53±15.77vs174.07±13.83,167.5±11.5,P<0.05);RT-PCR结果显示VEGF165转染后促进了BGC-823细胞MMP-2、u-PA的mRNA表达,Ad-VEGF165组MMP-2、u-PA的mRNA水平明显高于对照组及Ad-GFP组((MMP-2mRNA:0.6646±0.0486vs0.3803±0.0254,0.4096±0.0668;u-PAmRNA:0.8216±0.0798vs0.4043±0.0620,0.406±0.1158,均P<0.05).结论:经VEGF165转染后,胃癌细胞迁移能力增强,MMP-2、u-PA的mRNA表达增加,MMP-2、u-PA表达的增加可能为VEGF促进胃癌侵袭转移机制.AIM： To study the effects of vascular endothelial growth factor 165 （VEGF165） on invasion and metastasis of human gastric cancer cells and to explore its potential mechanism. METHODS： Human gastric cancer cell line, BGC-823 was cultured in vitro. BGC-823 was transfected with Ad-GFP and with Ad-VEGF165 separately. A millicell cell culture chamber assay in vftro was used to detect changes in migration capacity of BGC-823 cells. The influence of VEGF165 on the mRNA expression of matrix metalloproteinases-2 （MMP-2） and urokinase-type plasminogen activator（u-PA） was investigated using reverse transcription polyrnerase chain reaction （RT-PCR）. RESULTS： Migration assay showed that the migration rate of BGC-823 cells in Ad-VEGF165 group was significantly higher either than AdGFP or than control group（217.53 ± 15.77 vs 174.07 ± 13.83, 167.5 ± 11.5, P 〈 0.05）. PCR displayed that the VEGF165-transfected BGC-823 increased the expression of MMP-2 and u-PA mRNA, which was significantly higher in Ad-VEGF165 group than in Ad-GFP group or control group （MMP-2 mRNA： 0.6646 ± 0.0486 vs 0.4096 ± 0.0668, 0.3803 ± 0.0254; u-PA mRNA： 0.8216 ± 0.0798 vs 0.406 ± 0.1158, 0.4043 ± 0.0620, all P 〈 0.05）. CONCLUSION： VEGF165-transfected BGC-823 increases the tumor cells＇ migration capacity, and enhances the mRNA expression of MMP-2 and u-PA. VEGF promotes invasion and metastasis of gastric cancer possibly through upregulated expression of MMP-2 and u-PA.

关 键 词：血管内皮生长因子165 胃癌 迁移 转移 

分 类 号：R735.2[医药卫生—肿瘤] R743.33[医药卫生—临床医学]