肾癌细胞的增殖和凋亡状态  

INVESTIGATION OF CELL PROLIFERATIVE AND APOPTOTIC STATUSIN RENAL CELL CARCINOMA

作　　者：杨宝龙[1,2] 顾方六[1,2] 刘德祥[1,2] 宓培苏 晋伟[1,2] 孔祥田[1,2] 邓方明[1,2] 

机构地区：[1]北京医科大学第一医院泌尿外科研究所 [2]大连市中心医院泌尿外科

出　　处：《北京医科大学学报》1998年第1期9-11,共3页Journal of Peking University(Health Sciences)

摘　　要：目的：探讨肾癌的发生和发展机制。方法：采用免疫组织化学和原位缺口末端标记方法对４８例肾癌和３０例癌旁正常肾组织进行细胞增殖和凋亡状态的研究。结果：４８例肾癌组织增殖指数为３５．７９，对照组为２．２３，两组间经统计学分析差异非常显著（Ｐ＜０．００１）。４８例肾癌组织凋亡指数为１．２１，对照组为０．１６，两组间统计学分析差异非常显著（Ｐ＜０．０１）。并且增殖指数和凋亡指数均随肿瘤分级的增加而增高。４８例肾癌组织凋亡指数与增殖指数比值（０．０３５）明显低于对照组（０．１３），统计学分析差异显著（Ｐ＜０．０１），并随肿瘤分级的增加，凋亡指数与增殖指数比值逐渐减小。结论：肾癌组织中细胞凋亡处于抑制状态，细胞增殖的增加和凋亡的相对减少在肾癌的发生和发展中均起重要作用。Objective:To study the mechanisms of renal cell carcinoma(RCC). Methods: Immunohistochemistry and TUNEL (TdTmediated dUTPbiotin nick end labeling) were used on 48 cases of RCC and 30 cases of normal tissue near the tumors mentioned above RCC sections to detect cell proliferative and apoptotic status. Results: Proliferative indexes (PI) from the tumor (35.79) and normal tissues (2.23) show statistical differences, P<0.001, and apoptotic indexes (AI) also differ, tumors with 1.21, normal tissues with 0.16, P<0.01. Both PI and AI increase as tumor progresses. The ratio of AI and PI is lower in tumor tissue than in normal tissue, P<0.05, the ratio decreases as tumor progresses. Conclusion: Apoptosis in tumor tissue is inhibited. Higer cellular PI and lower AI play a key role in renal malignant development.

关 键 词：肾肿瘤 肾细胞癌 病理学 增殖 

分 类 号：R737.110.2[医药卫生—肿瘤]