骨巨细胞瘤中p53,c-fos和c-Abl基因蛋白的分布及意义  

Distribution and significance of p53, cfos and cAbl oncogene protein in giant cell tumor of bone

作　　者：刘斌[1] 王文亮[1] 张惠中[1] 赵一岭[1] 王剑波[1] 

机构地区：[1]第四军医大学基础部病理学教研室

出　　处：《第四军医大学学报》1998年第1期45-47,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金

摘　　要：目的：为了了解ｐ５３，ｃｆｏｓ及ｃＡｂｌ三种癌基因产物在骨巨细胞瘤中的表达情况，探讨基因改变与骨巨细胞瘤复发、恶变等生物学行为间的关系．方法：采用免疫细胞化学方法对９８例存档骨巨细胞瘤标本进行了检测．结果：①ｐ５３，ｃｆｏｓ和ｃＡｂｌ三种癌基因产物在骨巨细胞瘤组织中均有表达，阳性率分别为３９８％，４９０％及７５５％．阳性细胞散在或呈片状、团块状分布．本组病例中，全部阴性者１２例（１２２％），全部阳性者２１例（２１４％），仅ｐ５３阳性者７例（７１％），仅ｃｆｏｓ阳性者１例（１０％），仅ｃＡｂｌ阳性者２８例（２８６％），ｐ５３和ｃｆｏｓ阳性者２例（２０％），ｐ５３和ｃＡｂｌ阳性者８例（８１％），ｃｆｏｓ和ｃＡｂｌ阳性者２６例（２６５％）．②三种癌基因产物在骨巨细胞瘤Ⅰ，Ⅱ，Ⅲ级三组不同组织分级中均有表达，ｐ５３和ｃｆｏｓ三组间无显著性差异；而ｃＡｂｌ在Ⅱ级与Ⅲ级间有显著性差异（Ｐ＜００５）．③三种癌基因产物阳性表达在骨巨细胞瘤有、无复发组之间，有、无局部侵袭或（和）转移组之间，均无显著性意义．④ｐ５３与ｃｆｏｓ之间阳性率无显著性意义（Ｐ＞００５），而ｃＡｂｌ?Aim: To investigate the relation between p53, cfos and cAbl protein expression and biologic behavior of giant cell tumor of bone(GCT) Methods: Ninetyeight paraffin embedded specimens were examined by immunohistochemical method Results: ① The positive expression of all three genes, p53, cfos and cAbl was observed in giant cell tumor and the positive rates were 388%, 490% and 755% respectively The positive cells were scattering or massive in distribution Twelve cases were negative and twentyone cases were positive for all three proteins Seven, one and twelve cases were positive respectively for p53, cfos and cAbl only Two cases were positive for both p53 and cfos Eight cases were positive for both p53 and cAbl Twentysix cases were positive for both cfos and cAbl ② The positive expressions of all three genes were seen in giant cell tumor with different histological grades There were no remarkable significance among the three histological grades in p53 and cfos gene, but there was remarkable significance between histological grade II and III in cAbl(P<005) ③The positive expression of three oncogenes was no significant in giant cell tumor between with and without recurrence group, and between with and without local invasion or/and metastasis group ④ The positive rates were no significance between p53 and cfos, but were remarkable significant between cAbl and cfos as well as between cAbl and p53 Conclusion: The results suggest that p53, cfos and cAbl are involved in tumorgenesis and development of giant cell tumor with different biological effects and cAbl may play a more important role

关 键 词：骨肿瘤 骨巨细胞瘤 癌基因 P53 免疫组织化学 

分 类 号：R738.1[医药卫生—肿瘤]