绿豆和花生的超弱发光  

Ultraweak Biophoton Emission in Mung Bean and Peanut

作　　者：李德红[1] 邢达[1] 谭石慈[1] 王维江[1] 

机构地区：[1]华南师范大学激光生命科学研究所,广州510631

出　　处：《植物生理学报（0257-4829)》1998年第2期177-182,共6页Acta Phytophysiologica Sinica

基　　金：国家教委归国留学人员基金;华南师范大学青年基金

摘　　要：对黄化绿豆幼苗先形态建成过程的超弱发光图象（延迟发光）的初步观察发现：见光培养40min后的绿豆幼苗即可探测到明显的延迟发光；见光时间越长，光诱导的延迟发光强度也越强。从绿豆和花生幼苗的趋弱发光图象来看，生长健壮的幼苗发光较强。其中茎尖和新生幼叶的延迟发光最强，上胚轴、子叶和下胚轴弯钩较强，下胚轴伸长部分次之，根部发光最弱。从不同发育阶段叶片的超弱发光图象来看，光合作用较强、新陈代谢旺盛的成熟叶片的超弱发光较强；光合作用和其它代谢过程相对较弱的叶片（伸展叶、老叶和幼叶），其超弱发光强度相应较弱。而叶绿素提取液和失活绿叶观测不到超弱发光。此外，对超弱发光光谱的初步研究表明它很可能来自光合作用中叶绿素的发光。这些都暗示，植物的（诱导）超弱发光与光形态建成和光合作用等生长代谢过程密切相关。This paper studied the ultraweak biophoton emission (UBE) duringphotomorphogenesis of etiolated mungbeau seedlings in comparison with that ofpeanut seedlings by single photon counting system. The images of germinating mung beaus show that the light-inducedbiophoton emission is detectable after 40minutes of light exposure. The longer thebine in which seedlings were culturedunder light, the stronger the bioophoton emission was (Fig. 1 ), and stronger seedling produced stronger emission. Asfor the light emission in the investigatedplants, the strongest parts are the shootapex and newly-formed leaf, followed byhypercotyl , cotyledon and hypocotyl,and roots are the weakest one (Fig. 2).In respect to UBE of leaves at differentdevelopmental stages, mature leaf isstrongest, the maturing leaf and senescing leaf are weaker, and the young leafis weakest (Fig. 5). These suggest thatthe light-induced biophoton emission inplants may be closely related to the photomorphogenesis of etiolated seedlingsand photosynthesis and/or other physiological-biochenical reactions of plants.Moreover, significant UBE was not observed either in extract of chlorphyⅡ orin quickly- boiled- to- death young leaves(Figs. 3, 4A). The UBE spectrometry ofleaves and chlorophyⅡare characterizedby similar featares. These suggest thatthe UBE we observed is related to somemetabolic processes related to photosynthesis (Figs. 5, 4B). Further study mayexplain its characterishcs and biologicalmechanism.

关 键 词：生物超弱发光 光形态 光合作用 绿豆 花生 

分 类 号：Q945.11[生物学—植物学]