HcNPV半胱氨酸蛋白酶基因的核苷酸序列研究  

Nucleotide Sequence Analysis of the HcNPV Cysteine Protease Gene

作　　者：贡成良[1,2,3] 小林淳[1,2,3] 宫岛成寿 金伟[1,2,3] 吴祥甫[1,2,3] 

机构地区：[1]日本三重大学分子素材工学科 [2]浙江农业大学蚕学系 [3]中国科学院上海生物化学研究所

出　　处：《生物化学与生物物理学报》1998年第3期307-310,共4页

摘　　要：ＨｃＮＰＶ半胱氨酸蛋白酶基因的核苷酸序列研究贡成良１小林淳宫岛成寿金伟１吴祥甫２＊（日本三重大学分子素材工学科，三重５１４，日本；１浙江农业大学蚕学系，杭州３１００２９；２中国科学院上海生物化学研究所，上海２０００３１）关键词美国白蛾；核型多角体病毒...The cysteine protease (CP) gene of Hyphantria cunea nuclear polyhedrosis virus (HcNPV) has been located at the HindⅢ-3.5 kb fragment. The nucleotide sequence has been determined. The results indicated that its open reading frame of 975 nt encoded the protein of 324 amino acids. The baculovirus late gene promoter TAAG motif was identified at -24 nt upstream from the start codon ATG; The typical poly(A) signal sequence AATAAA was detected at 33 nt downstream from the stop codon TAA. The HcNPV CP exhibited 81%, 76% and 76% homology at the nucleotide level and 85%, 78%, 76% homology at the amino acid level to that of CfNPV (Choristoneura fumiferana nuclear polyhedrosis virus), AcNPV (Autographa californica nuclear polyhedrosis virus) and BmNPV (Bombyx mori nuclear polyhedrosis virus), respectively. HcNPV CP was a member of the papain superfamily and showed high homology to other cysteine protease, particularly, to those from Trypanosoma brucei and Dictyostelium discoideum with 31.8%, 34.5% identity at the amino acid level, respectively. Of 36 residues conserved among cathepsins B, H, L and S and papain, 32 were identical in HcNPV CP. The ERFNIN interspersed amino acid motif was found within the propeptide region of the HcNPV CP. The GlyCysAsnGlyGly motif conserved among all cysteine proteases was also detected in HcNPV CP.

关 键 词：核型多角体病毒 半胱氨酸 蛋白酶 核苷酸 序列 

分 类 号：Q939.42[生物学—微生物学]