SAMP8小鼠中枢皮质酮水平升高对兴奋性氨基酸含量的影晌  

INCREASED CORTICOSTERONE LEVEL AND ITS EFFECT ON CONTENT OF EXCITATORY AMINO ACIDS IN CEREBRAL CORTEX AND HIPPOCAMPUS IN SAMP8

作　　者：周建政[1] 张永祥[1] 周金黄[1] 

机构地区：[1]军事医学科学院毒物药物研究所

出　　处：《中国应用生理学杂志》1998年第2期117-119,共3页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金

摘　　要：用放射免疫法和高效液相色谱电化学检测器分别测定了ＳＡＭＰ８小鼠血浆、大脑皮层、海马、下丘脑皮质酮（ｃｏｒｔｉｃｏｓｔｅｒｏｎｅ，ＣＯＲＴ）水平和大脑皮层、海马兴奋性氨基酸含量。结果显示，ＳＡＭＰ８小鼠血浆ＣＯＲＴ水平均显著（Ｐ＜０．０５，或Ｐ＜０．０１）高于其同龄正常对照小鼠ＳＡＭＲ１。ＳＡＭＰ８小鼠８月和１２月龄大脑皮层和海马ＣＯＲＴ水平也明显（Ｐ＜０．０５，或Ｐ＜０．０１）高于同龄正常对照ＳＡＭＲ１小鼠。同时，ＳＡＭＰ８小鼠大脑皮层和海马谷氨酸（ｇｌｕ－ｔａｍａｔｅ，Ｇｌｕ）含量有上升趋势，谷氨酰胺（Ｇｌｕｔａｍｉｎｅ，Ｇｌｎ）含量则明显高于ＳＡＭＲ１小鼠。ＳＡＭＰ８小鼠切除肾上腺后，大脑皮层和海马Ｇｌｕ含量呈下降趋势，而Ｇｌｎ含量则显著降低。给切除肾上腺的ＳＡＭＰ８小鼠补充ＣＯＲＴ（ｉｐ，１０ｍｇ／ｋｇ），大脑皮层和海马Ｇｌｕ和Ｇｌｎ水平均明显（Ｐ＜０．０５，或Ｐ＜０．０１）升高，而天冬氨酸（Ａｓｐａｒｔａｔｅ，Ａｓｐ）含量始终没有变化。结果提示，ＳＡＭＰ８小鼠大脑皮层和海马Ｇｌｕ和Ｇｌｎ水平升高是肾上腺依赖的。ＳＡＭＰ８小鼠大脑皮层和海马Ｇｌｕ和Ｇｌｎ含量升高可能与其大脑皮层和海马ＣＯＲＴ水平升高有?Plasma corticosterone (CORT) concentration in SAMP8 increased significantly ( P <0. 05, or P <0. 01 ) compared with age matched SAMR1 while the contents of CORT in both cerebral cortex and hippocampus were also markedly ( P <0. 05 ,or P <0. 01) greater in 8 month and 12 month old SAMP8 than those in age matched SAMR1. Meanwhile, contents of glutamate (Glu) in cerebral cortex and hippocampus had a trend to rise and contents of glutamine (Gln) increased pronouncedly in SAMP8 compared with those in SAMR1. However, adrenalectomy in SAMP8 led to significant reduction of Gln contents in cerebral cortex and hippocampus while Glu contents decreased without statistical significance. Supplementation with CORT to adrenalectomized SAMP8 could once again result in a great elevation in both Glu and Gln contents. The present results suggest that the elevated contents of Glu and Gln in cerebral cortex and hippocampus in SAMP8 are adrenal dependent and the elevation of CORT levels in both cerebral and hippocampus in SAMP8 may be responsible for the elevated contents of Glu and Gln.

关 键 词：SAMP8 皮质酮 兴奋性氨基酸 皮层 早老性痴呆 

分 类 号：R749.160.2[医药卫生—神经病学与精神病学]