多能硫杆菌RubisCO基因的亚克隆及各种启动子对其表达的影响  

SUBCLONING OF RIBULOSE 1,5 BISPHOSPHATE CARBOOX YGENASE/OXYGENASE GENE OF THIOBACILLUS VERSUTUS AND EXPRESSION IN ESCHERICHIA COLI

作　　者：刘振盈[1] 颜望明[1] 

机构地区：[1]山东大学微生物技术国家重点实验室

出　　处：《山东大学学报（自然科学版）》1998年第1期95-100,共6页Journal of Shandong University(Natural Science Edition)

基　　金：国家及山东省自然科学基金

摘　　要：将多能硫杆菌（Ｔｈｉｏｂａｃｉｌｕｓｖｅｒｓｕｔｕｓ）ＲｕｂｉｓＣＯ基因从两个方向亚克隆到ｐＢＲ３２２和ｐＫＫ２２３－３载体上，通过酶切的方法对各种质粒的插入方向进行了确证．并进一步对这两种插入方向的质粒在大肠杆菌（Ｅｓｃｈｅｒｉｃｈｉａｃｏｌｉ）中的表达情况进行了酶活性的测定，该基因片段在ｐＫＫ２２３－３载体的ｔａｃ启动子的启动下，表达活性比ｌａｃ启动子以及ｐＢＲ３２２载体上的Ｐ１和Ｐ２启动子高．The RubisCO gene of Thiobacillus versutus was subcloned to the vectors of pBR322 and pKK223 3 from two directions.The inserting direction of these plasmids had been determined by the way of restriction enzyme.The enzyme activity was expressed by the recombinant plasmids in E.coli had been assayed.The expression activity of the RubisCO gene is higher at the promotion of tac promoter (pKK223 3) than that of the lac, P 1 and P 2 promoters.

关 键 词：多能硫杆菌 RUBISCO 基因表达 亚克隆 启动子 

分 类 号：Q939.415[生物学—微生物学]