基于微芯片电泳的脱氧核糖核酸片段的浓缩和分离  

Preconcentration and separation of DNA fragments based on microchip electrophoresis

作　　者：徐中其[1] 廣川健[1] 

机构地区：[1]广岛大学工学研究科化学与化工系

出　　处：《色谱》2009年第1期102-106,共5页Chinese Journal of Chromatography

摘　　要：采用超负荷电动供给(electrokinetic supercharging,EKS)预浓缩技术,在微芯片电泳(MCE)上对脱氧核糖核酸(DNA)片段进行浓缩和分离。EKS是集合样品电动进样(EKI)和过渡等速电泳(tITP)的一种在线浓缩方法。研究表明:采用该方法后,在40.5mm长的单通道芯片上能够实现对低浓度样品的大量进样、浓缩和基线分离。在普通的紫外检测条件(检测波长为260nm)下,对DNA片段的平均检出限(S/N=3)约为0.07mg/L,仅为十字芯片上的微芯片电泳检出限的1/40。本文还对浓缩过程中的一些关键因素和定性分析进行了探讨。An online preconcentration method, electrokinetic supercharging （EKS） was used for the enrichment of DNA fragments based on microchip electrophoresis （MCE）. EKS is a process that combines electrokinetic injection （EKI） with transient isotachophoresis （tITP）. The results demonstrated that the large volume of low concentration sample could be introduced, preconcentrated, and eventually separated on a single channel microchip with the whole length of 40. 5 ram. The limit of detection （S/N = 3 ） of DNA fragments was around 0. 07 mg/L, effectively improved 40-fold by EKS preconcentration with the normal UV detection at 260 nm. Some important parameters for enhancing preconcentration and qualitative analysis were examined.

关 键 词：微芯片电泳 超负荷电动供给 电动进样 过渡等速电泳 DNA片段 

分 类 号：O658[理学—分析化学]