AcMNPV大立方形多角体突变株突变位点的证实  

Identification of the Mutation Site of AcMNPV Big Cubic Mutant

作　　者：林广云[1] 施先宗[1] 龙綮新[1] 余奇理[2] 

机构地区：[1]中山大学生物防治国家重点实验室 [2]香港大学动物系

出　　处：《中山大学学报（自然科学版）》1998年第2期61-64,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省自然科学基金

摘　　要：纯化了３株在共转染后形成正常多角体的病毒株，提取其病毒ＤＮＡ，并对其做了ＥｃｏＲⅠ、ＢｇｌⅡ酶切分析；克隆含多角体蛋白基因的ＥｃｏＲⅠＩ片段并进行序列测定，证实了此病毒株为回复突变株．Three strains of recombinant virus which produce normal polyhedra after cotransfection was plaque purified. Viral DNA was analyzed with Eco R Ⅰ and Bgl Ⅱ; The Eco RⅠ I fragments which cover the polyhedrin gene were cloned and sequenced.It is thus proved that these viruses were reverse mutant.

关 键 词：杆状病毒 多角体蛋白 回复突变 突变位点 ACMNPV 

分 类 号：Q939.44[生物学—微生物学]