戊四氮致癫痫大鼠不同脑区神经肽Y和亮氨酸-脑啡肽的表达及其意义  

Expression of the Neuropeptide Y and Leu-Enkephalin in the Brain of Pentylenetrazol-Induced Epileptic Rat Models.

作　　者：陈琅[1] 陈巧彬[1] 邹漳钰 林立芳[3] 沈晓丽[3] 陈新[1] 季晓林 

机构地区：[1]福建省立医院儿科,福建医科大学省立临床医学院病理科,福州350001 [2]福建省直机关医院神经科 [3]福建省心血管病重点实验室

出　　处：《中华妇幼临床医学杂志（电子版）》2009年第1期12-16,共5页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：福建省自然科学基金资助项目(Z0516068)~~

摘　　要：目的神经肽Y(neuropeptide Y,NPY)和亮氨酸-脑啡肽(leu-enkephalin,L-ENK)在癫痫发生、发展和转归中有着重要作用,本研究在戊四氮(pentylenetetrazol,PTZ)致癫痫大鼠模型中观察神经肽Y和亮氨酸-脑啡肽在不同脑区中的变化趋势,探讨神经肽Y、亮氨酸-脑啡肽与癫痫的关系。方法将清洁级近交系雄性SD大鼠50只分为实验组(A组,n=40)和对照组(B组,n=10)。A组大鼠腹腔注射戊四氮1次,剂量为50 mg/kg,B组腹腔注射同等容量生理盐水。A组大鼠腹腔注射戊四氮后,根据癫痫发作进行分级,0~1级(未发生惊厥)为7只(A1组,n=7),立即取脑处理;2级以上发作(大发作)为33只,随机于癫痫发作后立即取脑处理(A2组,n=10)及6 h(A3组,n=11),24 h(A4组,n=10),72 h(A5组,n=2)取脑处理。B组于注射生理盐水后5 min^10 min内取脑处理。采用放射免疫方法动态观察A,B两组大鼠脑组织的海马、中脑、纹状体和额叶中神经肽Y、亮氨酸-脑啡肽的改变;S-P免疫组化染色法染色,观察A,B两组大鼠海马CA1区神经肽Y、亮氨酸-脑啡肽的表达。结果A1组大鼠中脑、纹状体及额叶的神经肽Y含量较B组升高(P<0.05)。A,B两组海马CA1区神经肽Y含量比较,差异无显著意义(P<0.05)。癫痫大发作组(A2,A3和A4组)以上各部位神经肽Y含量均明显高于B组;动态观察结果显示,癫痫发作后,在中脑、海马、纹状体和额叶的神经肽Y含量明显增高,随后呈逐步下降趋势,癫痫发作后24 h,其含量与未发生惊厥的A1组神经肽Y含量比较,差异无显著意义(P>0.05)。S-P免疫组化染色显示,癫痫大发作后,海马神经肽Y的表达明显增加,但随着时间的推移而降低。应用戊四氮后,癫痫大发作大鼠较未出现大发作的大鼠,其额叶、海马、中脑及纹状体中亮氨酸-脑啡肽含量均明显升高,两组间比较,除中脑外,其余部位差异有显著意义(P<0.05);但大发作大鼠海马、中脑及纹状体亮氨酸-脑啡肽含量与B组比较Objective To evaluate the significant changes of the neuropeptide Y （NPY） and leu-enkephalin（L-Enk） in the brain of pentylenetetrazol （PTZ）induced rats in order to understand the relationship among the neuropeptide Y,leu-enkephalin and epilepsy. Methods Fifty male adult SD rats were randomly divided into the experimental group （group A, n=40, intraperitoneal pentylenetetrazol injection 50 mg/kg） and the control group （group B ,n= 10, intraperitoneal tales doses normal saline injection）. According to the modification of seizure activity, the group A was divided into nomseizure group （group A1, n=7） which was decapitated immediately and the seizure group（group A2-A5,n=33）. Rats of the seizure group were decapitated immediately （group A2, n=10）, at the sixth hour（group A3, n=11）, at the 24th hour（group A4, n=10） and at the 72th hour（group A5, n=2） after the seizure. Radioimmunoassay （RIA） and immunocytochemical techniques were used to evaluate the changes of the neuropeptide Y and leu enkephalin in mesencephalon, hippocampus, striatum, and frontal cortex. Results The level of the neuropeptide Y increased significantly in mesencephalon（P〈0. 05）, frontal cortex（P〈0.05）, and striatum （P〈0.01） of the group A in contrast to those of the group B, and no difference was found in hippocampus. The level of the neuropeptide Y in brains of the seizure group（group A2 - A5, n = 33） was significant higher than that of the group B. The level of the neuropeptide Y returned to the value of the non-seizure group at the 24th hour after the seizure. Both groups had an elevated level of the leu-enkephalin in mesencephalon, striatum, hippocampus and frontal cortex. No difference was found between the non-convulsive group and the convulsive group in the four brain areas above, but the leu-enkephalin content of hypothalamus in the convulsive group was higher than that in the non-convulsive group. After the convulsion, the level of the leu--enkephalin decreased. Conclusion

关 键 词：癫痫 戊四氮 脑组织 神经肽Y 亮氨酸-脑啡肽 

分 类 号：R742.1[医药卫生—神经病学与精神病学]