某些细胞因子在天疱疮皮损中的表达  

Expression of some cytokines in lesions of pemphigus

作　　者：马月眉[1] 马烈[1] 栗玉珍[1] 周航[1] 付冬然[1] 于淞[1] 

机构地区：[1]哈尔滨医科大学附属第二医院皮肤科,哈尔滨150086

出　　处：《中国麻风皮肤病杂志》2009年第1期24-26,共3页China Journal of Leprosy and Skin Diseases

摘　　要：目的：检测Th2类细胞因子在天疱疮皮损中的表达。方法：选用单克隆抗体CD4，CD8，CD25，CD54和CD124等，对40例天疱疮患者的皮损及皮损周围皮肤标本用S—P法进行免疫组化研究，同时以20例正常人皮肤组织为对照。结果：CD4^＋和CD8^＋的T细胞见于全部标本真皮浸润的单一核细胞内，且以CD4^＋的T细胞为主（P〈0．01），在表皮CD8^＋的T细胞在寻常型天疱疮和红斑型天疱疮比较有显著性差异（P〈0．01），77例病人显示CD25染色阳性，与皮损周围相比CD54在皮损处表达增多（P〈0．05）。某些CD124阳性细胞见于表皮及真皮。对照组中CD4^＋和CD8^＋T细胞稀少，CIM／CD8比值为1．4，在皮损及皮损周围的皮肤中CD25的表达无显著性差异（P〉0．05），而角质形成细胞不表达CD25。CD54的表达见于血管内皮细胞和真皮血管周围的单一核细胞，与皮损周围相比，皮损处CD54^＋增加（P〈0．05）。结论：细胞免疫在天疱疮的发病中有重要作用。Objective： To investigate the role of Th2 - like cytokines in pathogenesis of pemphigns. Methods： The expressions of monoclonal antitibodies CD4, CD8, CD25, CD54 and CD124 in skin lesions and perilesional skin of 40 patients with pemphigus were immunohistologically measured by S - P method. Normal skin from 20 healthy people was used as control. Results： CD4^＋ and CD8^＋ T cells were detected in the dermal mononuclear cells of all biopsy specimens, with predominant CD4^＋ T cells （P 〈 0.01）. In epidermis CD8^＋ T cells were more in pemphigus vulgaris than in pemphigus erythematous （ P 〈 0.01 ）. Twenty - seven patients showed positive staining for CD25 and the expression of CD54 was predominant in lesions （ P 〈 0.05）. Some positive cells of CD124 were detected in epidermis and dennis. CD4^＋ and CD8^＋ T cells were very few, and the ratio of CD4^＋/CD8^＋ was 1.4. There was no difference of CD25 between lesions and perilesions （ P 〉 0.05）, but the keratinocytes didn＇t express CD25. Expression of CD54^＋ was found in vascular endothelial cells and perivascular mononuclears and CD54^＋ cells were more predominant than that in perivascullar areas（ P 〈 0.05）. Conclusion： The cell - mediated immunity may play an important role in the pathogenesis of pemphigus.

关 键 词：细胞因子 天疱疮 角质形成细胞 

分 类 号：R739.41[医药卫生—肿瘤] R758.66[医药卫生—临床医学]