Raman spectroscopic identification of normal and malignant hepatocytes  

作　　者：郭建宇 杜冰 钱曼 蔡炜颖 王祖赓 孙真荣 

机构地区：[1]State Key Laboratory of Precision Spectroscopy(East China Normal University),Department of Physics,and Department of Biology,East China Normal University

出　　处：《Chinese Optics Letters》2009年第1期60-63,共4页中国光学快报（英文版）

基　　金：supported by the Shanghai Priority Academic Discipline(B408);the National Natural Science Foundation of China(No.10574046);the Program for Changjiang Scholars and Innovative Research Team in University;the Program for New Century Excellent Talents in University(NCET-04-0420 and 30800);the Doctoral Program of High Education(No.20050269011);the Shanghai Municipal Science Technology Commission(No.07DZ22025 and 06QH14003)

摘　　要：Raman spectroscopy has strong potential for providing non-invasion diagnosis of cancers. In this paper, micro-Raman spectroscopy is used to diagnose one most common liver cancer, hepatocellular carcinoma （HCC）. The statistical analyzes, including t-test, principal component analysis （PCA）, and linear discriminant analysis （LDA）, are performed on the Raman spectra of malignant and normal hepatocytes. The t-test-LDA results show that the 786- and 1004-cm^-1 bands of the malignant and normal hepatocytes are significantly different, and PCA-LDA results show an overall accuracy of 100% for the Raman spectroscopic identification of normal and malignant hepatocytes in our experiment.Raman spectroscopy has strong potential for providing non-invasion diagnosis of cancers. In this paper, micro-Raman spectroscopy is used to diagnose one most common liver cancer, hepatocellular carcinoma （HCC）. The statistical analyzes, including t-test, principal component analysis （PCA）, and linear discriminant analysis （LDA）, are performed on the Raman spectra of malignant and normal hepatocytes. The t-test-LDA results show that the 786- and 1004-cm^-1 bands of the malignant and normal hepatocytes are significantly different, and PCA-LDA results show an overall accuracy of 100% for the Raman spectroscopic identification of normal and malignant hepatocytes in our experiment.

关 键 词：Discriminant analysis Raman scattering Raman spectroscopy Spectrum analysis 

分 类 号：R735.7[医药卫生—肿瘤]