颌面部鳞癌细胞生物学特性及p21蛋白表达的定量分析  

Quantification and Assay of p21 protein protein in Maxillofacial Squamous Cell Carcinoma

作　　者：刘维贤[1] 高原侠[1] 何宏华[1] 管宇[1] 

机构地区：[1]中国医科大学第二附属医院口腔科

出　　处：《中国肿瘤临床》1998年第4期279-282,共4页Chinese Journal of Clinical Oncology

摘　　要：本文应用双抗体标记和流式细胞分析技术，对５５例颌面部鳞状上皮源性良恶性肿瘤及正常组织进行ＤＮＡ指数、增殖指数、Ｓ期细胞比例及ｐ２１蛋白的表达进行了定量分析。结果：正常皮肤和乳头状瘤均为二倍体，鳞癌异倍体率为６０％，并随病理分级增高而增高；增殖指数和Ｓ期细胞所占的百分比在这三种组织中均有显著性差异；正常组织ｐ２１蛋白的表达为阴性；乳头状瘤ｐ２１蛋白阳性表达率为３０％；鳞癌ｐ２１阳性表达率为７０％；鳞癌ｐ２１蛋白的阳性表达率和表达强度均显著高于其它两种组织。结论认为ｐ２１蛋白在良性肿瘤中也有部分阳性表达。但表达率显著低于恶性肿瘤。The DNA index, proliferation index, ratio of Sphase cells and p21 gene expression were analysed quantitatively by using dual antibody staining and flow cytometric technique in a total of 55 cases including normal bucal squamous epithelium 5,benign papilloma 10 and squmous cell carcinoma 40.The results showed that the normal tissue and papilloma were diploidy, and that heteroploidy in squamous carcinoma was found in 60% and rose　with　pathological grading.p21 gene protein expression was negative in normal tissues,but in papilloma and squamous carcinoma it became as frequent as 30% and 70% respectively . The frequency of positive p21 gene expression　 elevated paralleling thepathological grading of the tumor.There was a significant difference of p21 gene expression between the benign and the malignant tumors,and　it correlated with degree of malignancy. In conclusion, benign tumors although exhibit expression of p21 protein but much lower than in malignant tumors.It might be used for surveillance purpose in those diseases with likely malignant propensity rather than for defining grade of malignancy. 

关 键 词：鳞癌 P21 流式细胞术 颌面部肿瘤 

分 类 号：R739.810.2[医药卫生—肿瘤]