HPV-DNA与p53蛋白在食管肿瘤中的作用及其与病人预后的关系  

The Roles of HPVDNA and p53 Protein in Esophageal Cancerand Its Correlation with the Patients Prognosis

作　　者：金玲[1] 王文亮[1] 王建清[1] 

机构地区：[1]沈阳军区总医院病理科

出　　处：《中国肿瘤临床》1998年第3期194-196,共3页Chinese Journal of Clinical Oncology

摘　　要：为了解人类乳头瘤病毒基因（ＨＰＶ－ＤＮＡ）６、１１及１６型及ｐ５３蛋白在食管鳞癌中的作用及其与病人预后的关系，对６４例食管鳞癌标本用原位分子杂交法检测了ＨＰＶ－ＤＮＡ６、１１和１６型表型；用免疫组化法检测了ｐ５３蛋白。结果发现：６４例食管鳞癌中ＨＰＶ－ＤＮＡ总的检出率为６２．５％，感染率较高，但ＨＰＶ６、１１型和１６型之间的检出率无显著差异。ＨＰＶ６、１１型的阳性率和肿瘤细胞分化程度有关，高分化者检出率高，低分化者检出率低。ＨＰＶ１６型的检出率在淋巴结转移组中比无转移组高；在ＴＮＭ分期Ⅲ、Ⅳ期的检出率比Ⅱ期者高；病人存活１年以内组中的检出率比存活３年以上者高。结论表明感染ＨＰＶ１６型者预后不佳；ＨＰＶ与ｐ５３双阳性者预后不佳。Objective:To search for the roles of human papilloma virusDNA (HPV DNA) and p53 protein in esophageal squamous cell cancer and　its　correlation with the patients prognosis.Method:The HPVDNA 6/11 types　were　determined using the in situ molecular hybridization technique on 64 samples of esophageal squamous cell cancer.The p53 protein was determined using immunohistochemical method in the same samples mentioned above.Result:The total positive rate of HPVDNA detected was 62 .5% with a higher infection rate,whereas the　detected rate between HPV6/11 type and 16 type showed no significant　difference.The positive rate of HPV 6/11 type paralleled the differentiation degree of the tumor cells. The positive rate of　HPV 16 type was higher in the group with lymph node metastasis　and TNM stage III,IV,whereupon the patient survived shorter,less than one year.Patient without metastasis in TNM stage II survived more than 3 years.Conclusion:The prognosis was poor in patients　with　HPV infection　or expressions of p53.

关 键 词：食管肿瘤 DNA P53蛋白 预后 人乳头瘤病毒 

分 类 号：R735.107[医药卫生—肿瘤]